We narrowed to 15,384 results for: pET
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Plasmid#170376PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Cre-inducible expression of nuclear RFP.DepositorInsertCre inducible H2B RFP
UseAAVPromoterhuman SynapsinAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-U6-UL29-U3-UL8
Plasmid#166685PurposeTo express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (non-EGFP version).DepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY1(L106P)
Plasmid#166714Purposeexpresses AMOT(L106P) in mammalian cellsDepositorInsertAngiomotin p130 L106P (AMOT Human)
Tags2xHAExpressionMammalianMutationchanged leucine 106 to prolinePromoterCMV promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY1(A112K)
Plasmid#166715Purposeexpresses AMOT(A112K) in mammalian cellsDepositorInsertAngiomotin p130 A112K (AMOT Human)
Tags2xHAExpressionMammalianMutationchanged alanine 112 to lysinePromoterCMV promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS313_ATP3G919C
Plasmid#120255PurposeExpression of ATP3-7 in yeastDepositorInsertATP3 (ATP3 Budding Yeast)
ExpressionYeastMutationG919 changed to C; Alanine 307 changed to ProlinePromoterATP3Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS313_ATP3mut
Plasmid#120256PurposeExpression of ATP3-67 in yeastDepositorInsertATP3 (ATP3 Budding Yeast)
ExpressionYeastMutationIsoleucine 304 changed to Asparagine; Alanine 307…PromoterATP3Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xMS2 no wild type
Plasmid#158204PurposeCassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted form the oligo pool experiment. It does not contain the MS2-wt sequence, therefore QCP cannot bind it.DepositorInsert10 binding sites of MS2 without the WT sequence from oligo library experiment
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xQb no wild type
Plasmid#158205PurposeCassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.DepositorInsert10 binding sites of QB coat protein without the WT sequence based on the oligo library experiment
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT140_GalL_cCDA1-VRERBE3
Plasmid#145103PurposeExpressing base editor cCDA1-VRERBE3 in yeast cellsDepositorInsertcCDA1-VRERBE3
UseCRISPRExpressionYeastMutationspCas9(D10A/D1135V/G1218R/R1335E/T1337R)PromoterGalLAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT122_GalL_cCDA1-VQRBE3
Plasmid#145087PurposeExpressing base editor cCDA1-VQRBE3 in yeast cellsDepositorInsertcCDA1-VQRBE3
UseCRISPRExpressionYeastMutationspCas9(D10A, D1135V, R1335Q, T1337R)PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT113_GalL_cCDA1-xBE3
Plasmid#145079PurposeExpressing base editor cCDA1-xBE3 in yeast cellsDepositorInsertcCDA1-xBE3
UseCRISPRExpressionYeastMutationspCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2VF/PA-VSV
Plasmid#139351PurposeExpression of the double mutant of TYK2-V678F-P1104ADepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
TagsVSVExpressionMammalianMutationV362F-V678F-P1104APromoterCMVAvailable SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisA-HOXC6-2
Plasmid#110365PurposeMammalian expression of HisA-tagged HOXC6-2DepositorAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph20-Stg
Plasmid#133035PurposeExpresses Xph20-Stg in bacteriaDepositorInsertXph20-Stg
TagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph18-ETWV
Plasmid#133036PurposeExpresses Xph18-ETWV in bacteriaDepositorInsertXph18-ETWV
TagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph18-Stg
Plasmid#133037PurposeExpresses Xph18-Stg in bacteriaDepositorInsertXph18-Stg
TagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph15-ETWV
Plasmid#133038PurposeExpresses Xph15-ETWV in bacteriaDepositorInsertXph15-ETWV
TagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph0-ETWV
Plasmid#133039PurposeExpresses Xph0-ETWV in bacteriaDepositorInsertXph0-ETWV
TagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIG-TAT-Xph20-ETWV
Plasmid#133040PurposeExpresses TAT-Xph20-ETWV in bacteriaDepositorInsertXph20-ETWV
TagsHis8, TAT, and TEV protease cleavage siteExpressionBacterialPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-RPAIN-202
Plasmid#117360PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR RPAIN-202
UseLuciferaseTagsluciferaseExpressionMammalianPromoterMurine PGK-1Available SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only