We narrowed to 10,047 results for: pET
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Plasmid#158203PurposeCassette of 10 different binding sites with high affinity to both PP7 and Qb phage coats protein, as computed by the ML model.DepositorInsert10 binding sites with high affinity to PP7 and QB coat proteins based on the machine-learning model
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xQb no wild type
Plasmid#158205PurposeCassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.DepositorInsert10 binding sites of QB coat protein without the WT sequence based on the oligo library experiment
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
6WAT
Plasmid#162268PurposeBaculovirus expression for structure determinationDepositorAvailable sinceDec. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
6PED
Plasmid#162265PurposeBaculovirus expression for structure determinationDepositorAvailable sinceDec. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBEL2082
Plasmid#155162PurposeExpresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)DepositorInsert(MlaFΔ[248-269])-(6xHis-TEV-MlaB)
ExpressionBacterialMutationdeleted amino acids 248-269 in MlaFAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJS263
Plasmid#134141PurposeBacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)DepositorInsertSka1 MTBD-His (Y106G6H.15 Nematode)
ExpressionBacterialAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
phSOD1-L126X-N’His
Plasmid#117707Purposemutation on stability/solubility of SOD1DepositorInsertSOD1 (SOD1 Human)
TagsHis tag with Thrombin cleavage siteExpressionBacterialMutationL126XPromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
phSOD1-D101G-N’His
Plasmid#117704Purposemutation on stability/solubility of SOD1DepositorInsertSOD1 (SOD1 Human)
TagsHis tag with Thrombin cleavage siteExpressionBacterialMutationD101GPromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGH50
Plasmid#107254PurposeVipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.DepositorInsertvipp1-gfp-fkbp12
UseSuicide vector for destined for double homologous…TagsGFP and FKBP12ExpressionBacterialPromoternativeAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mCardinal
Plasmid#105798PurposeExpression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mCardinalExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mAmber
Plasmid#105792PurposeExpression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the C-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mAmberExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mCerulean
Plasmid#105790PurposeExpression of your protein of interest in fusion with cyan fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mCeruleanExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mCardinal-TEV
Plasmid#105781PurposeExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsmCardinal-TEVExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mAmber-TEV
Plasmid#105775PurposeExpression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the N-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsmAmber-TEVExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mCerulean-TEV
Plasmid#105773PurposeExpression of your protein of interest in fusion with cyan fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsmCerulean-TEVExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTetR89-Wasabi
Plasmid#86104PurposemWasabi reporter gene gated by temperature-sensitive TetR A89DDepositorInsertsTetRts
mWasabi
Tags6xHisExpressionBacterialMutationA89DPromoterptetA and ptetRAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
5J3U (TgPKA, 583.m05422)
Plasmid#79525PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert583.m05422
Tags6xHis and TEV cleavage siteExpressionBacterialPromoterT7-lacOAvailable sinceJune 15, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
5CZG (Tb427.10.7420)
Plasmid#79517PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTb427.10.7420
Tags6xHis and TEV cleavage siteExpressionBacterialPromoterT7-lacOAvailable sinceJune 15, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJK652
Plasmid#72470PurposeProduces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)DepositorInsertacetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
TagsHis6ExpressionBacterialMutationMutated histidine-233 to glycinePromoterT7Available sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK691
Plasmid#72381PurposeProduces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)DepositorInsertstationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
TagsHis6ExpressionBacterialPromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only