We narrowed to 25,554 results for: Nov;
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Plasmid#159536PurposeDelivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 KbDepositorInsertNme2Cas9 with single guide RNA cassette
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianPromoterU1aAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pDisplay-GRAB-rDA3h-IRES-EGFP-CAAX
Plasmid#208697PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h and a membrane-localized EGFP in mammalian cellsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA3h
ExpressionMammalianPromoterCMVAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-DmrB-DmrB-Ripk3-2A-mCherry-puro
Plasmid#231972PurposeTet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosisDepositorInsertRipk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)
UseLentiviralMutationWTAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGFP-hNET
Plasmid#193364Purposeexpression of human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cellsDepositorInserthuman Norepinephrine Transporter (SLC6A2 Human)
Tagsmonomeric GFP (mGFP)ExpressionMammalianPromoterCMVAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB-rDA3m
Plasmid#208708PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons in a cre-dependent mannerDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA3m
UseAAVPromoterhSynAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOGS539_haPD-1
Plasmid#226716PurposePlasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)DepositorInsertHigh affinity PD-1 microbody
TagsHA tag and Mating factor alpha secretion signalExpressionYeastPromoterpTEF1Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB-rDA2h-IRES-EGFP-CAAX
Plasmid#208695PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h and a membrane-localized EGFP in mammalian cellsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA2h
ExpressionMammalianPromoterCMVAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-DmrB-Casp8-2A-mCherry-puro
Plasmid#231971PurposeTet inducible expression of DmrB-Casp8 with mCherry to induce Casp8 apoptosisDepositorInsertCasp8 dimerizing construct with bi-cistronic mCherry (CASP8 Human)
UseLentiviralAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKL038 Lenti U6 dCas12a gRNA Puro mCherry
Plasmid#195546PurposedCas12a gRNA expression backboneDepositorInsertLenti U6- empty cassette_Direct repeat_puro_mcherry
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
ExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
W45A mutant of H3K9me3 biosensor
Plasmid#120808PurposeFRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
ExpressionMammalianMutationTryptophan 45 is mutated to Alanine 45 on HP1 dom…PromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX--DmrB-DmrB-Ripk3(deltaC)-2A-mCherry-puro
Plasmid#231973PurposeTet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosisDepositorInsertRipk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)
UseLentiviralAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-Halo,IRES-eGFP
Plasmid#164519PurposeAll-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneExpressionMammalianAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3
Plasmid#213969PurposeAAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinaseDepositorInsertShank3 sgRNA
UseAAV and CRISPRAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-rDA3mut
Plasmid#208706PurposeExpresses the genetically-encoded fluorescent dopamine (DA) control sensor GRAB_rDA3mut in neuronsDepositorInsertGPCR activation based dopamine (DA) control sensor GRAB_rDA3mut
UseAAVPromoterhSynAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Td-CGBE
Plasmid#196599PurposeMammalian expression of Td-CGBEDepositorInsertbpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
Tags6*HisExpressionMammalianMutationTadA-8e (N46L); S. pyogenes Cas9 (D10A)Available SinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanCOL2A1enhancer
Plasmid#173961PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertEnhancer region from COL2A1 gene
UseLuciferaseMutationNonePromoterSV40 promoterAvailable SinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB-rDA2m-IRES-EGFP-CAAX
Plasmid#208694PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2m and a membrane-localized EGFP in mammalian cellsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA2m
ExpressionMammalianPromoterCMVAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW031
Plasmid#231144PurposeUsed to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.DepositorInsertS. aureus Sortase A mutant
TagsNostoc punctiform split intein C-terminal fragmen…ExpressionBacterialMutationP94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…PromoterT7Available SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only