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pTV-Ncapd3-tdiRFP670-PEST

Plasmid

#122362

Purpose

Targeting vector for mouse Ncapd3 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Ncapd3

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Npm1-tdiRFP670-PEST

Plasmid

#122363

Purpose

Targeting vector for mouse Npm1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Npm1

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Dnmt3b-tdiRFP670-PEST

Plasmid

#122364

Purpose

Targeting vector for mouse Dnmt3b to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Dnmt3b

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Dhx15-tdiRFP670-PEST

Plasmid

#122365

Purpose

Targeting vector for mouse Dhx15 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Dhx15

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Birc6-tdiRFP670-PEST

Plasmid

#122366

Purpose

Targeting vector for mouse Birc6 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Birc6

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Trim28-tdiRFP670-PEST

Plasmid

#122367

Purpose

Targeting vector for mouse Trim28 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Trim28

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Gsk3b-tdiRFP670-PEST

Plasmid

#122368

Purpose

Targeting vector for mouse Gsk3b to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Gsk3b

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Cdt1-tdiRFP670-PEST

Plasmid

#122369

Purpose

Targeting vector for mouse Cdt1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Cdt1

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Ddx42-tdiRFP670-PEST

Plasmid

#122370

Purpose

Targeting vector for mouse Ddx42 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Ddx42

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Klf4-tdiRFP670-PEST

Plasmid

#122350

Purpose

Targeting vector for mouse Klf4 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Klf4

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-mHes1-tdiRFP670-PEST

Plasmid

#122352

Purpose

Targeting vector for mouse Hes1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Hes1

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Peg3-tdiRFP670-PEST

Plasmid

#122353

Purpose

Targeting vector for mouse Peg3 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Peg3

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Rad21-tdiRFP670-PEST

Plasmid

#122354

Purpose

Targeting vector for mouse Rad21 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Rad21

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Ssrp1-tdiRFP670-PEST

Plasmid

#122355

Purpose

Targeting vector for mouse Ssrp1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Ssrp1

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Trim27-tdiRFP670-PEST

Plasmid

#122356

Purpose

Targeting vector for mouse Trim27 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Trim27

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCas9n-Nanog-L

Plasmid

#122302

Purpose

Expresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Nanog (Nanog Synthetic)

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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Prham-M.AvaIII-p15A-aadA

Plasmid

#149342

Purpose

expresses M.AvaIII DNA methyltransferase (M.NsiI ortholog) in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

Insert

M.AvaIII

Expression

Bacterial

Promoter

rhamnose

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pXF90

Plasmid

#139044

Purpose

MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

halotag::HDEL (includes STOP codon, PATC introns)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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DYNLRB2

Plasmid

#132534

Purpose

Human Rbl-2, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLRB2 (DYNLRB2 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCas9FnCpf1GG

Plasmid

#131013

Purpose

Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Inserts

direct repeat of SpCas9
GFP expression cassette
direct repeat of FnCas12a

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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