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pTIR_HyPB_RFP

Plasmid

#248201

Purpose

HyPB RFP cargo plasmid

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

RFP

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pTIR_Antgra4_RFP

Plasmid

#248203

Purpose

Antgra4 RFP cargo plasmid

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

RFP

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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excision_reporter_HyPB

Plasmid

#248200

Purpose

HyPB and synthetic transposase excision reporter and targeted integration reporter

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

Excision RFP reporter

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCSCG-Fc-pST3Gal1 H302A

Plasmid

#228820

Purpose

Expresses Fc-fusion pST3Gal1 H302A in mammalian cells

Depositor

Sriram Neelamegham

Article

Hombu et al Nat Commun. 2025 Jul 18;16(1):6637. doi:

Insert

Fc-pST3Gal1 H302A

Tags

6His tag and human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-59 of pST3Gal1 were deleted and H30…

Promoter

CMV

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pPD0053_pIVTRup_EBFP2

Plasmid

#242544

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

EBFP2

Use

Other

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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NanoLuc-A AND T-SpyTag

Plasmid

#246946

Purpose

Expresses multifunctional construct for AND-gated release of NanoLuciferase (NanoLuc) from materials in response to sortase eSrtA(2A9) AND TEV treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199

Insert

Please see depositor comments below

Tags

6x His tag, SsrA

Expression

Bacterial

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF1_HTC3xF_Puro_donor

Plasmid

#246395

Purpose

PHF1 donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF1 (PHF1 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_MTF2_sgRNA

Plasmid

#246402

Purpose

Cas9/sgRNA expression plasmid targeting MTF2

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

MTF2 (MTF2 Human)

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_PHF1_sgRNA

Plasmid

#246403

Purpose

Cas9/sgRNA expression plasmid targeting PHF1

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF1 (PHF1 Human)

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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CAaNa::GFP

Plasmid

#140507

Purpose

Encodes GFP downstream of an attP/attB flanked Neomycin stop cassette driven by the CAG promoter

Depositor

Mark Emerson

Article

Schick et al Sci Rep. 2019 Jun 27;9(1):9358. doi: 10.

Insert

attP-Neomycin-PolyA-attB

Use

Chicken expression

Expression

Mammalian

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-32

Plasmid

#246315

Purpose

Evaluation of VvU6.1 promoter (Pol III promoter) for CRISPR-Cas9 editing of mEGFP in Nicotiana benthamiana and poplar reporter lines

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

VvU6.1 promoter

Use

CRISPR

Expression

Plant

Promoter

VvU6.1 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_E11-YPet-H2B

Plasmid

#244034

Purpose

anchoring anti mTFP1DARPin_E11 fused to YPet to chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-YPet-HIST1H2BJ

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_G01-YPet-CAAX

Plasmid

#244033

Purpose

anchoring anti mTFP1DARPin_G01 fused to YPet to plasma membrane

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-YPet-CAAX

Tags

CAAX

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_E11-YPet-CAAX

Plasmid

#244032

Purpose

anchoring anti mTFP1DARPin_E11 fused to YPet to plasma membrane

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-YPet-CAAX

Tags

CAAX

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_G01-YPet-H2B

Plasmid

#244035

Purpose

anchoring anti mTFP1DARPin_G01 fused to YPet to chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-YPet-HIST1H2BJ

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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VN1897 pB2Hws_v28_Ddcm_wkKanR_TIR51_pLWeakRBS_TIR371_cI-Asf1B+rpoA-2GG

Plasmid

#235119

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B. In fusion cloning to rpoA using BsaI based golden gate.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Type

Empty backbone

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P

Expression

Bacterial

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-SlmiR482bTS-B/c

Plasmid

#234368

Purpose

Cloning syn-tasiRNA sequences in minimal SlmiR482bTS precursors

Depositor

Alberto Carbonell

Article

Tomassi et al Plant Biotechnol J. 2025 Oct 14. doi: 10

Inserts

B/c
SlmiR482bTS

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR2-3A

Plasmid

#245789

Purpose

Expression of mutated bovine arrestin-2 (I386A, V387A, F388A) in bacteria

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-2 (ARRB2 Bovine)

Expression

Bacterial

Mutation

I386A, V387A, F388A

Promoter

lac

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBtA3

Plasmid

#245782

Purpose

Expression of bovine arrestin-3 tagged with SmBiT in mammalian cells

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-3 (ARR3 Bovine)

Tags

SmBiT

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pTrcHIS2-MtFAAH1

Plasmid

#243679

Purpose

Expresses Medicago truncatula FAAH1 isoform in E. coli.

Depositor

Kent Chapman

Article

Arias-Gaguancela et al Sci Rep. 2023 May 9;13(1):7486. doi: 10.

Insert

Medicago truncatula Fatty Acid Amide Hydrolase 1

Tags

c-myc, 6xHIS

Expression

Bacterial

Promoter

trc

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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