We narrowed to 168,189 results for: addgene

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BRCA1 A10.2 gRNA

Plasmid

#90549

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA1 (Guide Designation A10.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158972

Purpose

Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMBEC6

Plasmid

#187598

Purpose

Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

nCas9-BEC-UGI; cas6f, gfp

Expression

Bacterial

Promoter

trc

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pWN_U6-TRAC-miniGag

Plasmid

#228960

Purpose

miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDT-SuperFi-Cas9

Plasmid

#183630

Purpose

Expression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coli

Depositor

David Taylor

Article

Bravo et al Nature. 2022 Mar 2. pii: 10.1038/s41586-

Insert

SuperFi-Cas9

Use

CRISPR

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …

Promoter

T7

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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BRCA2 A11.2 gRNA

Plasmid

#90550

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA2 (Guide Designation A11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-Cre

Plasmid

#50935

Purpose

retroviral expression of Cre recombinase

Depositor

Pier Pandolfi

Article

Chen et al Nature. 2005 Aug 4;436(7051):725-30.

Insert

Cre recombinase

Use

Cre/Lox and Retroviral

Tags

NLS

Expression

Mammalian

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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TAL3216

Plasmid

#42679

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-atg12-left (atg12 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pFGC5941-PacI-ath-STTM832-5p

Plasmid

#84163

Purpose

target miRNA832-5p for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM832-5p

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM832-3p

Plasmid

#84162

Purpose

target miRNA832-3p for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM832-3p

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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