We narrowed to 10,965 results for: T7
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Plasmid#198689PurposePeriplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)DepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody ON10
Tags6xHis tag, HA tag, OmpA signal peptide, and Ribos…ExpressionBacterialPromoterT7 promoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINQ-NbH4
Plasmid#198690PurposePeriplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)DepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody H4
Tags6xHis tag, Avitag, OmpA signal peptide, and Ribos…ExpressionBacterialPromoterT7 promoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1 (322-366)
Plasmid#193038PurposeBacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.DepositorAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1 L328D, L355A (1-366)
Plasmid#193040PurposeBacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.DepositorAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CAPN7 L61D (1-165)
Plasmid#193033PurposeBacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutationDepositorInsertCAPN7 (CAPN7 Human)
Tags6xHis-SumoExpressionBacterialMutationL61D, Residues 1-165PromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CAPN7 V18D,F98D (1-165)
Plasmid#193034PurposeBacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.DepositorInsertCAPN7 (CAPN7 Human)
Tags6xHis-SumoExpressionBacterialMutationV18D, F98D, Residues 1-165PromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1, residues 316-366, C-Cys
Plasmid#191234PurposeBacterial expression for IST1 residues 316-366, contains non-native C-terminal Cysteine for labelingDepositorInsertIST1 (IST1 Human)
TagsHIS-SUMOExpressionBacterialMutationResidues 316-366, contains nonnative C-terminus f…PromoterT7Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4A 153-222
Plasmid#108256PurposeExpresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6DepositorInsertCHMP4A residues 153-222 (CHMP4A Human)
TagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4C 156-233
Plasmid#108257PurposeExpresses C-terminal 156-233 residues of CHMP4C with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-9DepositorInsertCHMP4C residues 156-233 (CHMP4C Human)
TagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP1A 140-196
Plasmid#104593PurposeExpresses C-terminal 140-196 residues of CHMP1A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-1DepositorInsertCHMP1A residues 140-196 (CHMP1A Human)
TagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP2A 152-222
Plasmid#104594PurposeExpresses C-terminal 152-222 residues of CHMP2A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-3DepositorInsertCHMP2A residues 152-222 (CHMP2A Human)
TagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP2B 141-213
Plasmid#104595PurposeExpresses C-terminal 141-213 residues of CHMP2B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-4DepositorInsertCHMP2B residues 141-213 (CHMP2B Human)
TagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP3 159-222
Plasmid#104599PurposeExpresses C-terminal 159-222 residues of CHMP3 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-5DepositorInsertCHMP3 residues 159-222 (CHMP3 Human)
TagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SLC25A46[R257A,R347A]-P2A-Electra1 (pAH36)
Plasmid#227620PurposeOverexpression of SLC25A46 with R247A and R347A mutations in mammalian cell linesDepositorInsertSLC25A46 (SLC25A46 Human)
TagsP2A-mElectra1ExpressionMammalianMutationR257A,R347APromoterCMV, T7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14)
Plasmid#227619PurposeOverexpression of SLC25A46 lacking its N-terminal region in mammalian cell linesDepositorInsertSLC25A46 (SLC25A46 Human)
TagsP2A-mElectra1ExpressionMammalianMutationdeleted aa 2-83PromoterCMV, T7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG8Ha-His-EWSR1-359-549
Plasmid#195868PurposeExpresses the RRM-RG2-ZnF (359-549) of EWSDepositorAvailable SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-DXO
Plasmid#177993PurposeFor purification of N-terminally His-tagged DXO from bacteriaDepositorAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57 mini-FLAG-HA-Regnase-3 D252N
Plasmid#222652PurposeFor in vitro transcription of mouse Zc3h12c D252N mutant tagged with FLAG-HA at N-termDepositorInsertZc3h12c (Zc3h12c Mouse)
UseIn vitro transcriptionTagsFLAG and HAMutationmutated aspartic acid 252 to asparagine to abroga…PromoterT7Available SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57 mini-FLAG-HA-Regnase-3 WT
Plasmid#222651PurposeFor in vitro transcription of mouse Zc3h12c tagged with FLAG-HA at N-termDepositorAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 WT
Plasmid#222649PurposeFor in vitro transcription of mouse Zc3h12a tagged with FLAG-HA at N-termDepositorAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only