We narrowed to 31,956 results for: Eng

1. EKAREN5-gl

   Plasmid

   #225958

   Purpose

   The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.

   Depositor

   Toru Hiratsuka

   Article

   Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

   Insert

   EKAREN5-gl
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Promoter

   EF-1-alpha promoter

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQAT43

   Plasmid

   #223415

   Purpose

   T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for monocot plants; TTTV PAM; LbCas12a-D156R and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-LbCas12a-D156R-ZmUbi-crRNA scaffold
   

   Use

   CRISPR; T-dna vector

   Tags

   NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTK_Cerulean_Nanotag_70_neg_Control

   Plasmid

   #130568

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   negative oligo sequence
   

   Use

   Enhancer cloning nanotag reporter vector

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.R_TRE_minPro

   Plasmid

   #211517

   Purpose

   Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

   Depositor

   Justin English

   Article

   Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   SV40 and minPro

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tol2-krt4-GFP

   Plasmid

   #97151

   Purpose

   label apical epithelial cells in zebrafish

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   AcGFP
   

   Use

   Zebrafish

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.R_TRE_minTK

   Plasmid

   #211516

   Purpose

   Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

   Depositor

   Justin English

   Article

   Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   SV40 and minTK

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEB-Tg

   Plasmid

   #28220

   Depositor

   Linzhao Cheng

   Article

   Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

   Insert

   SV40 large T
   

   Expression

   Mammalian

   Available Since

   April 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. single cut NH-donor

   Plasmid

   #83575

   Purpose

   eGFP reporter donor containing single SA cutting site for NHEJ knock-in

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   eGFP
   

   Use

   Nhej donor

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQAT56

   Plasmid

   #223428

   Purpose

   T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. BPK2660

    Plasmid

    #70709

    Purpose

    Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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