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WEE1 F10.4 gRNA

Plasmid

#90941

Purpose

3rd generation lentiviral gRNA plasmid targeting human WEE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

WEE1 (Guide Designation F10.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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WEE1 F9.4 gRNA

Plasmid

#90940

Purpose

3rd generation lentiviral gRNA plasmid targeting human WEE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

WEE1 (Guide Designation F9.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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UBE2C H5.2 gRNA

Plasmid

#90934

Purpose

3rd generation lentiviral gRNA plasmid targeting human UBE2C

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

UBE2C (Guide Designation H5.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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TRIP13 H3.2 gRNA

Plasmid

#90920

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIP13

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TRIP13 (Guide Designation H3.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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PLK1 G6.1 gRNA

Plasmid

#90835

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PLK1 (Guide Designation G6.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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CDC6 B6.1 gRNA

Plasmid

#90597

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDC6 (Guide Designation B6.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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CDC6 B5.1 gRNA

Plasmid

#90596

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDC6 (Guide Designation B5.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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ECT2 D6.1 gRNA

Plasmid

#90673

Purpose

3rd generation lentiviral gRNA plasmid targeting human ECT2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ECT2 (Guide Designation D6.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23 D637G 2014 EBOV + Mucin-Like Domain

Plasmid

#86018

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Changed aspartic acid 637 to glycine

Promoter

CMV IE

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMT-myl7-NLS-BirA-2A-mCherry_Ras

Plasmid

#80061

Purpose

Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Myl7 promoter

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK8 gRNA (BRDN0001146407)

Plasmid

#78048

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK8 (MAPK8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK8 gRNA (BRDN0001147976)

Plasmid

#78049

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK8 (MAPK8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EXOSC10 gRNA (BRDN0001487044)

Plasmid

#77988

Purpose

3rd generation lentiviral gRNA plasmid targeting human EXOSC10

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EXOSC10 (EXOSC10 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ALDH18A1 gRNA (BRDN0001145444)

Plasmid

#77995

Purpose

3rd generation lentiviral gRNA plasmid targeting human ALDH18A1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ALDH18A1 (ALDH18A1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ZAP70 gRNA (BRDN0001487050)

Plasmid

#77956

Purpose

3rd generation lentiviral gRNA plasmid targeting human ZAP70

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ZAP70 (ZAP70 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ZAP70 gRNA (BRDN0001145290)

Plasmid

#77957

Purpose

3rd generation lentiviral gRNA plasmid targeting human ZAP70

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ZAP70 (ZAP70 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRIM24 gRNA (BRDN0001147238)

Plasmid

#77889

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM24

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM24 (TRIM24 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CCL2 gRNA (BRDN0001147096)

Plasmid

#77868

Purpose

3rd generation lentiviral gRNA plasmid targeting human CCL2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CCL2 gRNA (CCL2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AURKA gRNA (BRDN0001148015)

Plasmid

#77726

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AURKA (AURKA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AURKA gRNA (BRDN0001147803)

Plasmid

#77727

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AURKA (AURKA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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