We narrowed to 16,364 results for: puromycin

1. pLCHKO_HPRT1_exon3_2_As

   Plasmid

   #155058

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLCHKO_HPRT1_exon2_2_As

   Plasmid

   #155056

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLCHKO_HPRT1_exon2_1_As

   Plasmid

   #155055

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLCHKO_HPRT1_exon2_2_Lb

   Plasmid

   #155052

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-XYLT2-sgRNA

   Plasmid

   #154862

   Purpose

   Lentiviral expression of Cas9 and sgRNA targeting XYLT2

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   XYLT2 sgRNA (XYLT2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEBTetD-SNAP-Cep70

   Plasmid

   #136822

   Purpose

   Mammalian expression of the centrosomal protein Cep70 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep70 (CEP70 Human, Synthetic)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEBTetD-SNAP-CPAP

   Plasmid

   #136816

   Purpose

   Mammalian expression of the centrosomal protein CENPJ N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-CENPJ (CENPJ Human, Synthetic)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEBTetD-SNAP-Cep76

   Plasmid

   #136814

   Purpose

   Mammalian expression of the centrosomal protein Cep76 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep76 (CEP76 Human, Synthetic)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEBTetD-SNAP-α-tubulin

   Plasmid

   #136792

   Purpose

   Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-TUBA (DNMBP Human, Synthetic)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONOR-SNCAe2-WT

    Plasmid

    #85845

    Purpose

    Donor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFP

    Depositor

    Jens Schwamborn

    Article

    Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

    Insert

    SNCA exon 2 homology arms (SNCA Human)
    

    Use

    Tags

    -

    Expression

    Bacterial and Mammalian

    Mutation

    -

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPRv2-Snrnp40

    Plasmid

    #134255

    Purpose

    Lentivector for Snrnp40 CRISPR knockout

    Depositor

    Bruce Beutler

    Article

    Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

    Insert

    Snrp40 (Snrnp40 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Snrnp40 gRNA “GATAACTATGCGACGTTGAA”

    Promoter

    U6

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. miR290-SE-RGM-tdTomato targeting vector

    Plasmid

    #133047

    Purpose

    Targeting vector of RGM-tdTomato with homologous arms around Mir290 super-enhancer DMR

    Depositor

    Rudolf Jaenisch

    Article

    Song et al Mol Cell. 2019 Aug 15. pii: S1097-2765(1

    Insert

    miR290-SE-5arm-RGM-miR290-SE-3arm
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLPC-Flag-SOCS1-Y80D

    Plasmid

    #129516

    Purpose

    Retroviral vector for the expression of human SOCS1 with a Y80D mutation and a FLAG tag in N-terminal

    Depositor

    Gerardo Ferbeyre

    Article

    Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

    Insert

    SOCS1 (SOCS1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 80 to Aspartic acid

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPC-Flag-SOCS1-Y80F

    Plasmid

    #129517

    Purpose

    Retroviral vector for the expression of human SOCS1 with a Y80F mutation and a FLAG tag in N-terminal

    Depositor

    Gerardo Ferbeyre

    Article

    Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

    Insert

    SOCS1 (SOCS1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 80 to Phenylalanine

    Promoter

    cmv

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAPM-D4 miR30-AGO1 ts2

    Plasmid

    #115851

    Purpose

    AGO1 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    AGO1 shRNA (AGO1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-U6gRNA5(Apc-g1)-PGKpuroBFP-W

    Plasmid

    #105022

    Purpose

    Lentiviral gRNA plasmid targeting mouse Apc , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Apc (Apc Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKLV2-U6gRNA5(Slc25a51-g1)-PGKpuroBFP-W

    Plasmid

    #105028

    Purpose

    Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Slc25a51 (Slc25a51 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKLV2-U6gRNA5(Slc25a51-g2)-PGKpuroBFP-W

    Plasmid

    #105029

    Purpose

    Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Slc25a51 (Slc25a51 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(Pou5f1-g1)-PGKpuroBFP-W

    Plasmid

    #105035

    Purpose

    Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Pou5f1 (Pou5f1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV2-U6gRNA5(Kcmf1-g1)-PGKpuroBFP-W

    Plasmid

    #105017

    Purpose

    Lentiviral gRNA plasmid targeting mouse Kcmf1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Kcmf1 (Kcmf1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge