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pLPAR1_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101116

Purpose

Encodes dCas9(N) fused to LPAR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

LPAR1-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pNR280

Plasmid

#79579

Purpose

A118 attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pNR279

Plasmid

#79578

Purpose

BxbI attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR287

Plasmid

#79580

Purpose

TP901 attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pBNJ26.3

Plasmid

#66876

Purpose

For CFP tagging via transposon insertion.

Depositor

Thomas Hughes

Article

Sheridan et al BMC Biotechnol. 2004 Aug 18;4:17.

Insert

eCFP in a transposon

Use

Source for a tn7 transposon

Tags

eCFP

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSARSF53-259

Plasmid

#61757

Purpose

NpuDnaB(delta283)dC39 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

NpuDnaB(delta283)dC39 intein

Tags

H6-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSLfa PUb TAL-KMO-L

Plasmid

#52887

Purpose

Left hand TALEN against Aedes aegypti KMO gene

Depositor

Zach Adelman

Article

Aryan et al PLoS One. 2013;8(3):e60082. doi: 10.1371

Insert

TALEN-KMO

Promoter

Ae aegypti polyubiquitin

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pSLfa PUb TAL-KMO-R

Plasmid

#52888

Purpose

Right hand TALEN against Aedes aegypti KMO gene

Depositor

Zach Adelman

Article

Aryan et al PLoS One. 2013;8(3):e60082. doi: 10.1371

Insert

TALEN-KMO

Promoter

Ae aegypti polyubiquitin

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRiboS BsaHind

Plasmid

#36253

Depositor

Carolyn Bertozzi, Jessica Seeliger

Article

Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

Type

Empty backbone

Use

Synthetic Biology; Mycobacteria replicating vector

Expression

Bacterial

Promoter

Modified Hsp60

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pST39-His/Vps27(345-440) - Hse(275-375)

Plasmid

#17638

Depositor

James Hurley

Article

Prag et al Dev Cell. 2007 Jun . 12(6):973-86.

Insert

His/Vps27(345-440) – Hse1(275-375) (VPS27, HSE1 Budding Yeast)

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only