Addgene

We narrowed to 6,032 results for: WUS;

Showing: 1441 - 1470 of 6032 results

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pCAG-mRhubarb720

Plasmid

#197212

Purpose

Expresses the protein of mRhubarb720 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb720

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET-mKelly1

Plasmid

#119782

Purpose

Expresses mKelly1 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mKelly1

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9N

Plasmid

#80934

Purpose

Plasmid that expresses Cas9N in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pKeratin-miRFP713-N1

Plasmid

#197235

Purpose

Expresses the fusion protein of Keratin-miRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Keratin-miRFP713

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9C

Plasmid

#80939

Purpose

Plasmid that expresses Cas9C in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pH2B-LSSmKate2-N1

Plasmid

#31903

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

H2B-LSSmKate2

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Promoter

CMV

Available since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pCytERM-miRFP680-N1

Plasmid

#197183

Purpose

Expresses the fusion protein of CytERM-miRFP680 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-miRFP680

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDM_noro_A2_lacZA

Plasmid

#118819

Purpose

T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ alpha subunit reporter.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

Norovirus toehold switch A2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTC201B

Plasmid

#52193

Purpose

for creating reversible targeting construct

Depositor

Tian Chi

Article

Chaiyachati et al BMC Biol. 2012 Nov 30;10:96. doi: 10.118

Insert

promoter-trap cassette Neo-Ires-GFP

Use

Cre/Lox

Tags

Neo, GFP

Expression

Mutation

Promoter

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pRSCgfp-hAIM2

Plasmid

#51666

Purpose

Express GFP and hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Inserts

AIM2 (AIM2 Human)
GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and RSV

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pAE03

Plasmid

#61044

Purpose

To construct C-terminal GFP fusions, single crossover integration vector

Depositor

Jan-Willem Veening

Article

Eberhardt et al Mol Microbiol. 2009 Oct;74(2):395-408. d

Type

Empty backbone

Use

Synthetic Biology

Tags

GFP+

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCytERM-emiRFP670-N1

Plasmid

#197180

Purpose

Expresses the fusion protein of CytERM-emiRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-emiRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-His6

Plasmid

#51536

Purpose

Express His6-tagged mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

Promoter

lac operator

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pBAD-O-GECO1

Plasmid

#113938

Purpose

Bacterial expression of orange fluorescent Ca2+ sensor O-GECO1

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

O-GECO1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hAIM2-T7

Plasmid

#51538

Purpose

Express T7-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP713-HO1

Plasmid

#197255

Purpose

Expresses the protein of miRFP713 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP713

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-BREP

Plasmid

#172340

Purpose

AAV transfer plasmid for BREP (bioluminescent red protein)

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BREP

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTubulin-iRFP670

Plasmid

#197237

Purpose

Expresses the fusion protein of Tubulin-iRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Tubulin-iRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCytERM-SNIFP-N1

Plasmid

#197189

Purpose

Expresses the fusion protein of CytERM-SNIFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-SNIFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wmCardinal

Plasmid

#197251

Purpose

Expresses the protein of wmCardinal in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmCardinal

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-GAL4QF

Plasmid

#61309

Purpose

Expresses chimeric transactivator GAL4_DBD:QF2w_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

GAL4_DBD::QF2w_AD

Use

Tags

Expression

Insect

Mutation

Changed EK on C-terminus of QF2 to KKKK

Promoter

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLSSmKate1-Tubulin-C1

Plasmid

#31902

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

alpha-tubulin

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCytERM-smURFP-N1

Plasmid

#197178

Purpose

Expresses the fusion protein of CytERM-smURFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-smURFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-NLS-LSSmKate1

Plasmid

#31873

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS-LSS-mKate1

Use

Retroviral

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

Available since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pDM_noro_A2_lacZ

Plasmid

#118820

Purpose

T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ reporter.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

Norovirus toehold switch A2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
UBQ10:sXVE:S10-(MCS)

Plasmid

#108177

Purpose

Binary vectors used for the inducible expression of tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

UBQ10:sXVE:

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRSM42

Plasmid

#113942

Purpose

Constitutive bacterial expression of red fluorescent Ca2+ sensor jRGECO1a

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jRGECO1a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDM_noro_S2_lacZA

Plasmid

#118816

Purpose

T7 RNAP-driven expression of norovirus sense orientation toehold switches (S2) with a lacZ alpha subunit reporter.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

Norovirus toehold switch S2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDM_noro_S6_lacZA

Plasmid

#118817

Purpose

T7 RNAP-driven expression of norovirus sense orientation toehold switches (S6) with a lacZ alpha subunit reporter.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

Norovirus toehold switch S6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
KA1390_pGL4-UBCmin-luc2

Plasmid

#124185

Purpose

Luciferase reporter with the UBC minimal promoter

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge