Addgene

We narrowed to 4,082 results for: Erf

Showing: 961 - 980 of 4082 results

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pKM692

Plasmid

#134224

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 235-509

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKM673

Plasmid

#134222

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C 510-end

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSL1211

Plasmid

#129521

Purpose

pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

SpCas9

Use

Plasmodium, rodent-infectious species

Tags

3XFlag-NLS

Expression

Mutation

Promoter

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-TAK1

Plasmid

#117162

Purpose

Target site AGCGCCCTTCAATGGAGGAAAT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-IRF5

Plasmid

#117157

Purpose

Target site TATTTCCCTGTCTCCTTGGCC

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-IRF7

Plasmid

#117158

Purpose

Target site ATAAGGAAGCACTCGATGTCG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-STAT2

Plasmid

#117161

Purpose

Target site TTTAAGTTCCACAGACTTGGA

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a - TryX

Plasmid

#89623

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi tryparedoxin protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Tryparedoxin

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCas73

Plasmid

#82398

Purpose

Δccm:Km, used to delete ccm operon

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

ccm deletion

Use

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-pcr1P-natMX6-pcr2P

Plasmid

#80620

Purpose

to delete part of the mat1-P locus and fix the mating type in natural S. pombe isolates

Depositor

Miguel Godinho Ferreira

Article

Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

Inserts

PCR1 from mat1-P
PCR2 from mat1-P

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-pcr1M-natMX6-pcr2M

Plasmid

#80621

Purpose

to delete part of the mat1-M locus and fix the mating type in natural S. pombe isolates

Depositor

Miguel Godinho Ferreira

Article

Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

Inserts

PCR1 from mat1-M
PCR2 from mat1-M

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 RRN3

Plasmid

#67112

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

RRN3 (RRN3 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TFAP4

Plasmid

#67073

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFAP4 (TFAP4 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

P338A

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pAN-PBAD-sgRNA-VR

Plasmid

#62286

Purpose

expression of VR sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

VR sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PA5-RFP

Plasmid

#62251

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

pA5

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pQTEV-CGI12

Plasmid

#34800

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

CGI-12 (MTERF3 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pSG5-p85α-ΔiSH2-C

Plasmid

#39499

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 ΔiSH2-C (PIK3R1 Bovine)

Use

Eukaryotic

Tags

Expression

Mutation

N488I, ΔiSH2-C = deletion of AA494-582

Promoter

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
Kek1 (Lip1) clone #1

Plasmid

#38750

Depositor

Norbert Perrimon

Article

Musacchio et al Dev Biol. 1996 Aug 25;178(1):63-76.

Insert

Kek1 (kek1 Fly)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only
M15 pCS-twhh-nBeta gal

Plasmid

#17152

Depositor

Randall Moon

Article

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

twhh promoter (shhb Zebrafish)

Use

Tags

nuclear beta-gal

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pRBC-sfGFP-150TAG

Plasmid

#174076

Purpose

Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

Depositor

Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Insert

Superfolder GFP

Use

Tags

His-6

Expression

Bacterial

Mutation

N150TAG

Promoter

T7

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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