We narrowed to 58,905 results for: SAP

1. pJV129

   Plasmid

   #124253

   Purpose

   In vitro transcription plasmid for T7 transcription of KRAS G12V sequence targeted to the endosome. Linearize with SapI.

   Depositor

   Stanley Riddell

   Article

   Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

   Insert

   Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. APP_pCSdest

   Plasmid

   #53877

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   APP (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

3. PX458_ARID4B_1

   Plasmid

   #101078

   Purpose

   Encodes gRNA for 3' target of human ARID4B

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against ARID4B (ARID4B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Kv7.4 BD (522-645, C643A)/pET28HMT

   Plasmid

   #111073

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   His Tag and MBP tag

   Expression

   Bacterial

   Mutation

   Kv7.4 BD (522-645, C643A)

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. Kv7.4 (1-645, Δ368-492)/pcDNA3.1

   Plasmid

   #111454

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-492), C643A

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1-FLAG-SF3B1-WT

   Plasmid

   #82576

   Purpose

   Expresses a codon-optimized ORF of human SF3B1 (wild-type)

   Depositor

   Manoj Pillai

   Article

   Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

   Insert

   human SF3B1-WT (SF3B1 Human)
   

   Use

   Tags

   FLAG peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. T7_CC_PE7_IVT_Template

   Plasmid

   #223022

   Purpose

   Template for in vitro transcription of PE7. For HSPC-related experiments.

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Template for in vitro transcription

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAX APP Swe/Ind

   Plasmid

   #30145

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   human APP 695 Swedish/Indiana mutation (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Swedish and Indiana; K595N, M596L and V642F

   Promoter

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-CAAX

   Plasmid

   #86056

   Purpose

   A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Homo sapiens Harvey rat sarcoma (HRAS Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains the region from 825-857 of HRAS as a far…

   Promoter

   CMV

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUGW-APPSwe-T2A-mCherry

    Plasmid

    #190804

    Purpose

    Express Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Article

    Ma et al J Neurosci. 2023 Mar 8;43(10):1830-1844.

    Insert

    APP (APP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Swedish (KM670/671NL)

    Promoter

    Ubi C

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti HsATP13A3 D498N

    Plasmid

    #195850

    Purpose

    Expresses D498N mutant Homo sapiens ATP13A3

    Depositor

    Veerle Baekelandt

    Article

    Houdou et al Biomolecules. 2023 Feb 9;13(2):337. doi:

    Insert

    ATPase 13A3 D498N (ATP13A3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSin-EF2-Sox2-Pur

    Plasmid

    #16577

    Depositor

    James Thomson

    Article

    Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

    Insert

    Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSin-EF2-Oct4-Pur

    Plasmid

    #16579

    Depositor

    James Thomson

    Article

    Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

    Insert

    Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag Depdc5 pRK5

    Plasmid

    #46340

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Differs from Depdc5 isoform 1 by 10 amino acids …

    Promoter

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSin-EF2-LIN28-Pur

    Plasmid

    #16580

    Depositor

    James Thomson

    Article

    Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

    Insert

    Homo sapiens lin-28 homolog (C. elegans) (LIN28A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-Sac-Abeta(MC1-42)

    Plasmid

    #127151

    Purpose

    Expresses N-terminal cysteine Abeta(1-42)

    Depositor

    James Nowick

    Article

    Zhang et al Biochemistry. 2021 Apr 20;60(15):1191-12

    Insert

    Abeta(MC1-42) (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. hZMPSTE24-3FLAG_pQCXIP

    Plasmid

    #89759

    Purpose

    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.

    Depositor

    Martin Dorf

    Article

    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127

    Insert

    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    

    Use

    Retroviral; Bicistronic expression: puromycin re…

    Tags

    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…

    Expression

    Mammalian

    Mutation

    Y253H in ZMPSTE24 (see depositor comments below)

    Promoter

    CMV

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF1a-hMLH1dn (og codon)

    Plasmid

    #174823

    Purpose

    Mammalian expression of human MLH1dn (original codon usage)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (original codon usage) (MLH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of residues 754-756

    Promoter

    EF1a

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. LAMP-mChF-AIP

    Plasmid

    #61524

    Purpose

    Expression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIP

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    lysosomal-associated membrane protein 1 (LAMP1 Human)
    

    Use

    Tags

    AIP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-Sac-Aβ(M1-42/C18C33)

    Plasmid

    #173759

    Purpose

    Expresses the AβC18C33 peptide.

    Depositor

    James Nowick

    Article

    Zhang et al Biochemistry. 2022 Feb 15;61(4):252-264.

    Insert

    Aβ(M1-42/C18C33) (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed valine 18 to cysteine; changed glycine 33…

    Promoter

    T7

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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