We narrowed to 24,649 results for: fer

1. pBbdCas9S(-10AC12)_Psyn-sgRNArodA

   Plasmid

   #149661

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi rodA

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNArodA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30(-10AC12), Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPRIME-TREX-GFP-FF3

   Plasmid

   #11667

   Purpose

   3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Insert

   FF3
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

   Plasmid

   #149588

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi flaB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAflaB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbdCas9S(RBSmut)_Psyn-sgRNAftsI

   Plasmid

   #149590

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAftsI
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. cycLuc_SbMVs

   Plasmid

   #119209

   Purpose

   Circularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   cyclic luciferase with SbMVp cleavage site
   

   Use

   Luciferase

   Tags

   AU1 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. hCMV-IE1:Lucia:bgH

   Plasmid

   #135293

   Purpose

   Transcriptional unit for constitutive expression of Lucia luciferase in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:Lucia:bGH
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBbdCas9S(RBSmut)_Psyn-sgRNAmreB

   Plasmid

   #149594

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi mreB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAmreB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGREAT17

   Plasmid

   #170905

   Purpose

   GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   p35SoTMV

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGB1a2R+pNOS_Red-F_NOSt

   Plasmid

   #170888

   Purpose

   GoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Reverse orientation

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   Red-F
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   p.S286Y Red mutant

   Promoter

   pNOS

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGREAT4

    Plasmid

    #170892

    Purpose

    GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pG10-90

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Mut.Blimp1pGL3 (Mutant human Blimp1 promoter in pGL3-basic)

    Plasmid

    #40341

    Depositor

    Alexander Dent

    Article

    Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

    Insert

    Blimp1 promoter (mutated) (PRDM1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Two AP-1 sites at -1813 and -1647 replaced with B…

    Promoter

    Blimp1

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pBbdCas9S(RBSmut)_Psyn-sgRNArodA

    Plasmid

    #149656

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RAB14 3'UTR D1D2

    Plasmid

    #61492

    Purpose

    luciferase reporter containing RAB14 3' UTR with both predicted MiR144 binding site deleted

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    RAB14 3' UTR (RAB14 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    contains position 837-1637 (numbering relative to…

    Promoter

    PGK

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

14. RAB14 3'UTR D1

    Plasmid

    #61490

    Purpose

    luciferase reporter containing RAB14 3' UTR with the first predicted MiR144 binding site deleted

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    RAB14 3' UTR (RAB14 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    contains position 837-1637 (numbering relative to…

    Promoter

    PGK

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

15. RAB14 3'UTR D2

    Plasmid

    #61491

    Purpose

    luciferase reporter containing RAB14 3' UTR with the second predicted MiR144 binding site deleted

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    RAB14 3' UTR (RAB14 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    contains position 837-1637 (numbering relative to…

    Promoter

    PGK

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pUB_SM-FLuc0_EMCV-Ren

    Plasmid

    #210590

    Purpose

    Expresses SM(FLAG)-nonoptimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Inserts

    Spaghetti monster (FLAG)-nonoptimal FLuc
    Optimal Renilla luciferase
    

    Use

    Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    EMCV IRES and UBC human

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUB_SM-FLuc100_EMCV-Ren

    Plasmid

    #210591

    Purpose

    Expresses SM(FLAG)-optimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Inserts

    Spaghetti monster (FLAG)-optimal FLuc
    Optimal Renilla luciferase
    

    Use

    Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    EMCV IRES and UBC human

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGREAT11

    Plasmid

    #170899

    Purpose

    GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_AtACT2t+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pNOS

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGREAT15

    Plasmid

    #170903

    Purpose

    GoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_AtACT2t+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pCmYLCV911

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGREAT16

    Plasmid

    #170904

    Purpose

    GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pNOS

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge