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pCIneo-V5-SBP-CeSmg7-G80E_S

Plasmid

#147466

Purpose

Mammalian Expression of CeSmg7-G80E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7-G80E (smg-7 Nematode)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-CeSmg7-T83E_S

Plasmid

#147467

Purpose

Mammalian Expression of CeSmg7-T83E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7-T83E (smg-7 Nematode)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-CeSMG5_1-420_S

Plasmid

#147468

Purpose

Bacterial Expression of CeSMG5_1-420

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSMG5_1-420 (smg-5 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetMP-CeSmg7_1-395_S

Plasmid

#147475

Purpose

Bacterial Expression of CeSmg7_1-395

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7_1-395 (smg-7 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-CeSmg5-H116E_S

Plasmid

#147458

Purpose

Mammalian Expression of CeSmg5-H116E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg5-H116E (smg-5 Nematode)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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p005-RFP-weak

Plasmid

#108316

Purpose

Synthetic RFP based on Corynactis spp under weak synthetic constitutive promoter pFAB4024; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

RFP under controlf of weak synthetic constitutive promoter pFAB4024

Use

Tags

Expression

Bacterial

Mutation

Promoter

pFAB4024

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBP-BBa_J23100

Plasmid

#74097

Purpose

BBa_J23100 promoter (iGEM collection)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_J23100 constitutive promoter (Sigma70)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCas8

Plasmid

#82380

Purpose

dCas9 without a promoter in acsA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas73

Plasmid

#82398

Purpose

Δccm:Km, used to delete ccm operon

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

ccm deletion

Use

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-BBa_B0012

Plasmid

#72997

Purpose

BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_B0012 terminator

Use

Synthetic Biology

Tags

Expression

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-BBa_B0015

Plasmid

#72998

Purpose

BBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

BBa_B0015 terminator

Use

Synthetic Biology

Tags

Expression

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP_BBa_B0034

Plasmid

#72980

Purpose

Bba_B0034 iGEM RBS (5' GTAC/3' CATA fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_B0034 RBS

Use

Synthetic Biology

Tags

Expression

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TetOn-mCherry-eGFP-RAMP4

Plasmid

#109014

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-SEP_NR2B

Plasmid

#23998

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

NR2B (Grin2b Rat)

Use

Tags

NR2B signal sequence (aa1-31) and SEP (supereclip…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pK18sB

Plasmid

#177838

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

Depositor

Gregg Beckham, Christopher Johnson

Article

Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

Type

Empty backbone

Use

Bacterial gene replacement

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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Adeno Cas9

Plasmid

#64072

Purpose

Adenoviral vector for the expression of the Cas9

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Type

Empty backbone

Use

Adenoviral

Tags

FLAG

Expression

Mutation

Promoter

U6 and CBh

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-C1C2-EYFP

Plasmid

#35519

Depositor

Karl Deisseroth

Article

Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

Insert

ChR1-ChR2 Chimera

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

June 19, 2012

Availability

Academic Institutions and Nonprofits only

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pCI-SEP_NR2A

Plasmid

#23997

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

NR2A (Grin2a Rat)

Use

Tags

SEP

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

Plasmid

#63166

Purpose

GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-HA"

Use

Tags

N-myristoylation

Expression

Insect

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

Plasmid

#63168

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-V5"

Use

Tags

N-myristoylation

Expression

Insect

Mutation

Promoter

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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