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bdSUMO-E5TAT-GFP-HiBit

Plasmid

#170993

Purpose

For bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-E5TAT-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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ALFY-FYVE 1G

Plasmid

#197045

Purpose

Expression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Traffic. 2021 Jan;22(1-2):23-37. doi: 10

Insert

ALFY (WDFY3 Human)

Tags

6xHis-GST-TEV

Expression

Bacterial

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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bdSUMO-R9-GFP-HiBit

Plasmid

#170990

Purpose

For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-R9-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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ppih.001.177.W133A

Plasmid

#137655

Purpose

Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704

Insert

ppih (PPIH Human)

Tags

GST-(His)6-thrombin

Expression

Bacterial

Mutation

001-177-W133A

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_W403A_6xH

Plasmid

#185712

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed W403 to A

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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bdSUMO-5.3-GFP-HiBit

Plasmid

#171014

Purpose

For bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-5.3-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET30-SBL1(160E,HisTagDel)

Plasmid

#66879

Purpose

Expresses a version of pET30Xa-SBL1(160E) with 41 bp Histag removed

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160E)

Expression

Bacterial

Mutation

S160E

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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WT USP7 catalytic

Plasmid

#227201

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Article

Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

aa 207-560

Promoter

T7-promoter

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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HIS-HA-CMYC P53

Plasmid

#233738

Purpose

expresses HIS-HA-CMYC triple tagged P53 for pulldown assay

Depositor

Albert Keung

Article

Han et al Methods. 2025 Mar;235:92-99. doi: 10.101

Insert

HIS-HA-CMYC-p53 (TP53 Human)

Tags

HIS-HA-CMYC

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CARD8-TEV-GFP-FLAG

Plasmid

#169991

Purpose

Mammalian purification construct for CARD8

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

TEV-GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTarget

Plasmid

#186452

Purpose

used as source for acquisition of new spacers

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMW96

Plasmid

#178066

Purpose

Expression of His6-SUMO-TEV-GFP-PreScission-γ-TuNA in E. coli

Depositor

Tarun Kapoor

Article

Wieczorek et al Cell. 2020 Jan 9;180(1):165-175.e16. doi

Insert

CDK5RAP2 (CDK5RAP2 Human)

Expression

Bacterial

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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BirA_BVES untagged

Plasmid

#196581

Purpose

expresses BirA

Depositor

Cheryl Arrowsmith

Article

Alteen et al Structure. 2023 Sep 7;31(9):1121-1131.e6

Insert

BirA_Ecoli

Use

Baculovirus expression

Available Since

June 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pMS1088

Plasmid

#80159

Purpose

pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme

Depositor

Marcelo Sousa

Article

Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/

Insert

HisSUMO-Cys-4xNuG2-Ald

Tags

HisSUMO

Promoter

T7

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMS1251

Plasmid

#80163

Purpose

pET-Duet for bacterial expression of Ald-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme

Depositor

Marcelo Sousa

Article

Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/

Insert

HisSUMO-Ald-4xNuG2-Ald

Tags

HisSUMO

Promoter

T7

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pTarget_PS_VA

Plasmid

#186454

Purpose

pTarget containing protospacer for FnCas12a targeting

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTarget_priming_VA

Plasmid

#186455

Purpose

pTarget containing protospacer with a mismatch on position 1, for FnCas12a targeting

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTarget_PS_VB

Plasmid

#186456

Purpose

pTarget containing protospacer for AaCas12b targeting

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET30Xa-SBL1(160E)

Plasmid

#66846

Purpose

Expresses soybean lipoxygenase-1 S160E mutant with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (LOC547694, NEWENTRY Glycine max)

Tags

His-tag

Expression

Bacterial

Mutation

S160E

Promoter

T7lac

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pET30-SBL1(160S,HisTagDel)

Plasmid

#66880

Purpose

Expresses a version of pET30Xa-SBL1(160S) with 41 bp Histag removed

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160S)

Tags

none

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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