We narrowed to 14,422 results for: nts

1. psiCHECK-302a-1

   Plasmid

   #42088

   Depositor

   Kumiko Ui-Tei

   Article

   Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

   Insert

   ZEB1 (ZEB1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   contains the 4055-4078 nt segment of ZEB1 mRNA (N…

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. psiCHECK-640a-2

   Plasmid

   #42093

   Depositor

   Kumiko Ui-Tei

   Article

   Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

   Insert

   ZEB1 (ZEB1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   contains the 1635-1657 nt segment of ZEB1 mRNA (N…

   Available Since

   Feb. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. psiCHECK-215a-2

   Plasmid

   #42086

   Depositor

   Kumiko Ui-Tei

   Article

   Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

   Insert

   ZEB1 (ZEB1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   contains the 2444-2466 nt segment of ZEB1 mRNA (N…

   Available Since

   Feb. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGCP123-EbpA_g1

   Plasmid

   #153517

   Purpose

   sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

   Depositor

   Kimberly Kline

   Article

   Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

   Insert

   pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-B/c

   Plasmid

   #227962

   Purpose

   Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   B/c
   

   Promoter

   None

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCT310

   Plasmid

   #111140

   Purpose

   Bacterial expression of 6His-dCas9-3XFlag

   Depositor

   Robert Tjian

   Article

   Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

   Insert

   dCas9
   

   Tags

   3X Flag and 6X His

   Expression

   Bacterial

   Mutation

   D10A - H840A

   Promoter

   T7

   Available Since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF12J

   Plasmid

   #42943

   Depositor

   Eline Luning Prak

   Article

   Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

   Insert

   EN+ Human L1 element (L1RE1 Human)
   

   Tags

   EGFP (opposite strand)

   Expression

   Mammalian

   Mutation

   AgeI-BclI fragment swapped with nt 1927-3708 from…

   Promoter

   CMV MIE, L1 5'UTR

   Available Since

   April 25, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pdRBFC-NS1-YN

   Plasmid

   #74723

   Purpose

   Express C-terminal YN fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labeling

   Depositor

   Aiming Wang

   Article

   Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

   Insert

   Influenza A virus NS1 protein, partial
   

   Use

   Double-stranded rna labeling

   Tags

   HA and YFP N-terminal half domain

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdRBFC-NS1-YC

   Plasmid

   #74724

   Purpose

   Express C-terminal YC fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labeling

   Depositor

   Article

   Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

   Insert

   Influenza A virus NS1 protein, partial
   

   Use

   Double-stranded rna labeling

   Tags

   FLAG and YFP C-terminal half domain

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdRBFC-B2-YN

    Plasmid

    #74721

    Purpose

    Express C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling

    Depositor

    Aiming Wang

    Article

    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

    Insert

    Influenza A virus NS1 protein, partial
    

    Use

    Double-stranded rna labeling

    Tags

    HA and YFP N-terminal half domain

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pdRBFC-B2-YC

    Plasmid

    #74722

    Purpose

    Express C-terminal YC fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling

    Depositor

    Aiming Wang

    Article

    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

    Insert

    Influenza A virus NS1 protein, partial
    

    Use

    Double-stranded rna labeling

    Tags

    FLAG and YFP C-terminal half domain

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDuRCC-K

    Plasmid

    #81193

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below., Please view…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK233-hCaM

    Plasmid

    #47598

    Depositor

    Emanuel Strehler

    Article

    Rhyner et al Biochemistry. 1992 Dec 29;31(51):12826-3

    Insert

    CaM (CALM3 Human)
    

    Expression

    Bacterial

    Mutation

    contains 469 bp fragment (nt 92-559)

    Promoter

    trc

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGCP123-GFP_g1

    Plasmid

    #153518

    Purpose

    sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-mCherry-CyrCR1.mutant

    Plasmid

    #128746

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
    

    Expression

    Mammalian

    Mutation

    5 point mutations disrupting the miR-7 site

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Fireworks GFP[PTC-] (AVA2598)

    Plasmid

    #85445

    Purpose

    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Expression

    Mammalian

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFS462

    Plasmid

    #89065

    Purpose

    his7 integration plasmid with Z3EVpr:GFP

    Depositor

    Nick Rhind

    Article

    Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

    Insert

    Z3EV promoter GFP-NLS leu1 C-terminal 300 nt
    

    Use

    S. pombe his7 integration vector

    Promoter

    Z3EV

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Fireworks RFP[PTC+] (AVA2626)

    Plasmid

    #85444

    Purpose

    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Expression

    Mammalian

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDuRCC-N

    Plasmid

    #81194

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please view depositor comments below and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TL1 alphaL_DdCBE 1-2-3 (right, DddA11-N)

    Plasmid

    #221997

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE repeats (alphaL-1/2/3 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: S1330I, A1341V, N1342S, E1370K, T1380I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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