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pHes7-Achilles-Hes7

Plasmid

#153528

Purpose

Expresses fusion protein of Achilles/YFP and mouse Hes7 from mouse Hes7 promoter. The reporter shows oscillatory expression with 2-3 periodicity when expressed in mouse presomitic mesoderm.

Depositor

Ryoichiro Kageyama

Article

Yoshioka-Kobayashi et al Nature. 2020 Apr;580(7801):119-123. doi:

Insert

Hes7 (Hes7 Mouse)

Tags

Achilles

Expression

Mammalian

Promoter

mouse Hes7 promoter

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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tetO-ID1

Plasmid

#170692

Purpose

doxycycline-inducible overexpression of ID1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

ID1 (ID1 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-GATA6

Plasmid

#170688

Purpose

doxycycline-inducible overexpression of GATA6

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

GATA6 (GATA6 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_POU2F3_CR

Plasmid

#122175

Purpose

Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

Insert

POU2F3 (POU2F3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

3 silent mutations for gRNA resistance (882g-c, 8…

Promoter

EFS

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-hKCNQ3-P2A-EGFP

Plasmid

#209195

Purpose

Cre-dependent expression of Kcnq3 and GFP from the same transcript

Depositor

Larry Zweifel

Article

Gamal El-Din et al Proc Natl Acad Sci U S A. 2021 Nov 9;118

Insert

Kcnq3 (KCNQ3 Human)

Use

AAV

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2_IRES2_EGFP_KCNQ2 var4

Plasmid

#173154

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Promoter

Simian CMV IE94

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET-TRSter

Plasmid

#78865

Purpose

Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element

Depositor

Elias Arnér

Article

Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.

Insert

Thioredoxin Reductase (Txnrd1 Rat)

Tags

Mutated E. coli-type SECIS element (3’ end of ins…

Expression

Bacterial

Promoter

T7lac

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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MOM603

Plasmid

#133242

Purpose

expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

Depositor

Shinsuke Niwa

Article

Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

Insert

kif1a (KIF1A Human)

Tags

StrepII tag and mScarlet

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 HSPA8

Plasmid

#19514

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 8 (HSPA8 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

Plasmid

#51255

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248 mutated to Ala

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

Plasmid

#51257

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248,789,805,817,848,9…

Promoter

EF1a

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

Plasmid

#51256

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248 mutated to Glu

Promoter

EF1a

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pPD0035_CROPseq-CAR-Puro_CD19-28z

Plasmid

#242983

Purpose

CD19-28z FMC63 CAR

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

CD19-28z FMC63 CAR (CD19 Human)

Use

Lentiviral

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pEN019-SpCas9-hiNLS-t-M1M4

Plasmid

#234456

Purpose

Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Article

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M1M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLPC-L647RLaminA

Plasmid

#69060

Purpose

encodes a lamin A mutant with Leucine 647 mutated to an Arginin

Depositor

Gerardo Ferbeyre

Article

Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

Insert

LaminA (LMNA Human)

Use

Retroviral

Expression

Mammalian

Mutation

Lamin A with Leucine 647 mutated to Arginine

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-BHLHE40

Plasmid

#110154

Purpose

Expression of human BHLHE40 in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

Insert

BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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huCaV2.2 (pSAD442-1)

Plasmid

#62574

Purpose

Human CaV2.2 expression in mammalian cells

Depositor

Diane Lipscombe

Article

Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993

Insert

CACNA1B (CACNA1B Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Synapto-pHluorin

Plasmid

#168510

Purpose

Expression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

Synaptophysin-pHluorin (Syp Mouse)

Tags

pHluorin

Expression

Mammalian

Promoter

CAG

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLV V5-lamin A

Plasmid

#175107

Purpose

Lentiviral expression of V5-tagged mouse Lmna

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Lmna (Lmna Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK3 S368A

Plasmid

#122680

Purpose

Expresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)

Expression

Mammalian

Mutation

changed Serine 368 to Alanine

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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