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pFC31

Plasmid

#165845

Purpose

_C31 integrase CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

_C31 integrase

Expression

Insect

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pFRETgc-2in1-NN

Plasmid

#105124

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mEGFP/mCherry

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFRETgc-2in1-CN

Plasmid

#105118

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mCherry and mEGFP

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO C1-APEX2

Plasmid

#129641

Purpose

Express ER-localized C1-APEX2 fusion in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

C1(1-29)-APEX2-GFP

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMINTC3H

Plasmid

#110075

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and His10 tag.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBbA8c-RFP

Plasmid

#35272

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-GFP-rtTA

Plasmid

#32648

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GFP
rtTA

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2011

Availability

Academic Institutions and Nonprofits only
pMIG FLAG B56 gamma3

Plasmid

#13803

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B56 gamma 3 Flag (PPP2R5C Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
EcYbbN pET15b

Plasmid

#60690

Purpose

Recombinant protein expression of YbbN

Depositor

Mark Wilson

Article

Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

Insert

YbbN

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-2T Sam68

Plasmid

#17687

Depositor

David Shalloway

Article

Resnick et al Oncogene. 1997 Sep;15(11):1247-53.

Insert

Sam68 (Khdrbs1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pMiR-HPV16-E7

Plasmid

#53696

Purpose

Luciferase reporter assay for microRNA binding on HPV16 E7

Depositor

Edward Chan

Article

Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

Insert

HPV16-E7

Use

Luciferase

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only
ins-cre_cryaa-Venus

Plasmid

#24333

Depositor

Didier Stainier

Article

Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

Insert

Cre (cre )

Use

Cre/Lox

Tags

Venus

Expression

Worm

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pDmAlfaTub84b

Plasmid

#165781

Purpose

Drosophila Alpha Tubulin promoter

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

AlfaTub84b

Expression

Insect

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-GFP-ER(T2)CreER(T2)

Plasmid

#32649

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GFP
ER(T2)CreER(T2)

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only
pBAD*RFPEC2

Plasmid

#17823

Depositor

Jay Keasling

Article

Pfleger et al Biotechnol Bioeng. 2005 Dec 5. 92(5):553

Insert

E. coli optimized red flourescent protein (RFPec2)

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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pJJS009

Plasmid

#188332

Purpose

PCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

mIAA7::mKate2::Linker

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt16

Plasmid

#42291

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt16 (Wnt16 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP N1 Ub-CRT

Plasmid

#69619

Purpose

Express cytoplasmic mature CRT.

Depositor

Marta Hallak

Article

Goitea et al J Biol Chem. 2015 Jun 26;290(26):16403-1

Insert

Ubiquitina-Calreticulin (mature)

Tags

EGFP and Ubiquitin

Expression

Mammalian

Mutation

Deletion of signal peptide of calreticulin at the…

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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scFv-M-MLV-RT

Plasmid

#181809

Purpose

scFv-M-MLV RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

MMLV_RT

Use

Lentiviral

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJJS003

Plasmid

#188326

Purpose

PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

mIAA7::mCherry::Linker

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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