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pCAGEN_N167_eMagBF_V5_NES

Plasmid

#235622

Purpose

Encodes ALIBi N-terminal fragment, residues 1-167, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-167

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N195_eMagBF_V5_NES

Plasmid

#235624

Purpose

Encodes ALIBi N-terminal fragment, residues 1-195, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-195

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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B8-pVTCB052

Plasmid

#231302

Purpose

Plasmid used for expression of AI-designed GFP protein B8 in E. coli

Depositor

Alexander Rives

Article

Hayes et al Science. 2025 Feb 21;387(6736):850-858.

Insert

B8

Expression

Bacterial

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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env8-AD-5'antiPNA-U1

Plasmid

#233011

Purpose

U1 snRNA tagged with 1 copy of Riboglow (truncated variant A with 5' antiPNA motif)

Depositor

Amy Palmer

Article

Wierzba et al RSC Chem Biol. 2024 Dec 19. doi: 10.1039

Insert

U1 (RNU1-1 Human)

Expression

Mammalian

Mutation

1 copy of Riboglow tag (truncated variant A with …

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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p35S-FHA-SpAGO4a_gDNA

Plasmid

#216841

Purpose

N-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a gDNA under regulation of CaMV 35S promoter

Depositor

Arturo Marí-Ordóñez

Article

Dombey et al bioRxiv 2024.04.03.587901; doi: https://

Insert

ARGONAUTE4

Tags

Flag-HA

Expression

Plant

Promoter

CaMV 35S

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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p35S-FHA-SpAGO4a_cDNA

Plasmid

#216842

Purpose

N-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a cDNA under regulation of CaMV 35S promoter

Depositor

Arturo Marí-Ordóñez

Article

Dombey et al bioRxiv 2024.04.03.587901; doi: https://

Insert

ARGONAUTE4

Tags

Flag-HA

Expression

Plant

Promoter

CaMV 35S

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgMTHFD1

Plasmid

#217436

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human MTHFD1

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA targeting MTHFD1 (MTHFD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC2-TK2-3xFLAG

Plasmid

#217427

Purpose

Lentiviral overexpression of human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

TK2 (TK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert sequence is a codon-optimized gBLOCK (IDT)

Promoter

CMV

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgTK2_4

Plasmid

#217431

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 4 targeting TK2 (TK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgTK2_5

Plasmid

#217432

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 5 targeting TK2 (TK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgSHMT2

Plasmid

#217435

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human SHMT2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA targeting SHMT2 (SHMT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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CamKII-mtPyronicSF

Plasmid

#228906

Purpose

Expression of mtPyronicSF in neurons targeted to the mitochondria serving as an optical sensor for mitochondrial pyruvate uptake. Backbone from Addgene plasmid 100834

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

Insert

pyronic SF

Use

AAV

Tags

Cox8 targetting

Expression

Mammalian

Promoter

CamKII

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pIN3 (gpdA-cTerm-Citrine2-ptrA)

Plasmid

#188737

Purpose

Expresses Citrine2 for C-terminal tagging

Depositor

Michael Bromley

Article

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

gdpA-Citrine2 PtrA

Use

Filamentous fungal expression

Tags

Citrine2

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIN4 (gpdA-nTerm-Citrine2-ptrA)

Plasmid

#188738

Purpose

Expresses Citrine2 for N-terminal tagging

Depositor

Michael Bromley

Article

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

gdpA-Citrine2 PtrA

Use

Filamentous fungal expression

Tags

Citrine2

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIN5 (gpdA-cTerm-mTurqoise2-ptrA)

Plasmid

#188739

Purpose

Expresses mTurqoise2 for C-terminal tagging

Depositor

Michael Bromley

Article

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

gdpA-mTurquoise PtrA

Use

Filamentous fungal expression

Tags

mTurquoise

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIN6 (gpdA-nTerm-mTurqoise2-ptrA)

Plasmid

#188740

Purpose

Expresses mTurqoise2 for N-terminal tagging

Depositor

Michael Bromley

Article

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

gdpA-mTurqoise2 PtrA

Use

Filamentous fungal expression

Tags

mGreenlantern

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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2BT-His-TEV-cs-LC3b

Plasmid

#197460

Purpose

Bacterial Expression of human LC3b

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

LC3b (MAP1LC3B Human)

Tags

His

Expression

Bacterial

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCas3-Apr

Plasmid

#208000

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8

Expression

Mammalian

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-NS3/4A S139A

Plasmid

#186885

Purpose

Doxycycline-dependent expression of catalytically inactive NS3/4A protease gene (S140A) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

HCV NS3/4A protease with S139A mutation

Use

Retroviral

Mutation

S139A

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-WHG cGAS-HA

Plasmid

#186867

Purpose

Doxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

White-handed gibbon cGAS with C-terminal HA tag

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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