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pBbS6a-RFP

Plasmid

#35292

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pGAL80

Plasmid

#165825

Purpose

GAL4 inhibitor coding sequence (CDS)

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GAL80

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQS

Plasmid

#165826

Purpose

QF inhibitor coding sequence (CDS)

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

QS

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFF749

Plasmid

#61452

Purpose

IPTG-inducible msd_kanR(ON)_dRT

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_kanR(ON)_dRT

Use

Synthetic Biology

Mutation

D197A D198A

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pBbB3a-GFP

Plasmid

#35346

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProS

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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AByF

Plasmid

#111084

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Inserts

Y homology arm left
gypsy insulator
3xP3 promoter
tdTomato
SV40 3'UTR
CTCF insulator
Y homology arm right

Expression

Insect

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pWZ414-F31

Plasmid

#23067

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed lysine 14 to Glutamine K14Q

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pCMVd2-mCherry-Dr-TrkA

Plasmid

#127957

Purpose

Myr mCherryDrTrkA expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

Insert

Myr-mCherry-3xSAG-Dr-hel-4- TrkA

Expression

Mammalian

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET28 mSmo-Cterm

Plasmid

#58703

Purpose

used for anti-mouse Smoothened antibody production (for affinity purification)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Smo (Smo Mouse)

Tags

6X-HIS

Expression

Bacterial

Mutation

contains C terminus (aa 543-793)

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only
pDmActin5c

Plasmid

#165777

Purpose

Drosophila Actin 5C promoter

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Actin5c

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CK(1.3)GW

Plasmid

#27227

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

AAV

Tags

EGFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pcR295/527

Plasmid

#17679

Depositor

David Shalloway

Article

Bagrodia et al Nature. 1991 Jan 10. 349(6305):172-5.

Insert

c-src K295R Y527F (SRC Chicken)

Expression

Mammalian

Mutation

Lys 295 changed to Arg. Tyr 527 changed to Phe.

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pGL-Rbl1UTR

Plasmid

#20884

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

Rbl1 (p107) 3'UTR (Rbl1 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
GST cdc25c C330S C377S

Plasmid

#10970

Depositor

Toren Finkel

Article

Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

Insert

cdc25c C330S C377S (CDC25C Human)

Tags

GST

Expression

Bacterial

Mutation

C330S C377S. Cysteine to Serine substitution at …

Available Since

Nov. 23, 2005

Availability

Academic Institutions and Nonprofits only
pSuper 44-1

Plasmid

#51597

Purpose

shRNA 1 against rat and mouse Sema4D

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

shRNA1

Use

RNAi

Promoter

U6

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pBABE Rad S105N

Plasmid

#11214

Depositor

Ronald Kahn

Article

Zhu et al J Biol Chem. 1996 Jan 12. 271(2):768-73.

Insert

Rad (RRAD Human)

Use

Retroviral

Expression

Mammalian

Mutation

S105N

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
pD2-dcas9-J23101-GFP

Plasmid

#122260

Purpose

Constitutive dCas9 and GFP expression plasmid

Depositor

Chueh Loo Poh

Article

Zhang et al Sci Rep. 2018 Sep 3;8(1):13127. doi: 10.

Insert

dCas9

Use

Synthetic Biology

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJLI-sup35

Plasmid

#1210

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 5' and 3' flanking regions (SUP35 Budding Yeast)

Expression

Yeast

Mutation

sup35 gene is not included in this plasmid. Only…

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pFRETvr-2in1-NN

Plasmid

#105126

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mVenus/tagRFP

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHL 2057

Plasmid

#64909

Purpose

AmpR + ColE1 + PLtetO-1::RBS(st7):cro::cII (31 basepairs)::T1 terminator + PLlacO-1::RBS(st7):psu::T1 terminator

Depositor

Han Lim

Article

Hussein et al Biochim Biophys Acta. 2015 May 14;1849(8

Type

Empty backbone

Use

Synthetic Biology; E. coli

Expression

Bacterial

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only