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Showing: 9841 - 9860 of 14665 results

  3. pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN

    Plasmid
    #69586
    Purpose
    Expresses mutated p53-L344P tagged with mKate2 and split N-term mVenus
    Depositor
    Galit Lahav
    Publication
    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:
    Insert
    p53-L344P (TP53 Human)
    Use
    Lentiviral
    Tags
    mKate2 and split mVenus - N term
    Expression
    Mammalian
    Mutation
    p53 L344P mutation that prevents dimerization and…
    Promoter
    EF1alpha
    Available since
    April 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    69586-plasmid-map-sequence-id-135228

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  5. pBrain-GFP-TACC3KDP∆682-688-shTACC3

    Plasmid
    #69116
    Purpose
    Dual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
    Depositor
    Stephen Royle
    Publication
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deletion of amino acids 682-688 (region containin…
    Promoter
    CMV
    Available since
    Oct. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    69116-plasmid-map-sequence-id-122088

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  8. pBridge-tyrosinase.σ1A

    Plasmid
    #197381
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Publication
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-1 σ1A
    Tags
    GAL4-DNA binding domain fragment, HA tag, and SV4…
    Expression
    Yeast
    Mutation
    Contains an extra 42 nucleotides encoding 14 resi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197381-plasmid-map-sequence-id-388878

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  11. pET30-GBF-E6C

    Plasmid
    #21698
    Depositor
    James Baleja
    Publication
    Liu et al J Mol Biol. 2009 Mar 6. 386(4):1123-37.
    Insert
    Protein G domain B1 fused C-terminal zinc binding domain of human papillomavirus type 16 (residue 79-142) (E6 Human papillomavirus type 16)
    Tags
    Protein G B1 domain
    Expression
    Bacterial
    Mutation
    GB1 (1-56aa) fusion tag with mutations at three p…
    Available since
    Aug. 31, 2009
    Availability
    Academic Institutions and Nonprofits only

  17. GFP Cav2.2 human HAgHA -minus exon 18a -LONG-Cterm in pcDNA3

    Plasmid
    #198914
    Purpose
    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a - Long form of alternatively spliced C-teminus, in pcDNA3 vector
    Depositor
    Annette Dolphin
    Publication
    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cav2.2 (CACNA1B Human)
    Tags
    N-terminal GFP, internal double HA
    Expression
    Mammalian
    Mutation
    exon 18a deleted, exon 19 starts 3 nucleotides ea…
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    198914-plasmid-map-sequence-id-392117

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  18. pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter

    Plasmid
    #172597
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.
    Depositor
    Daniel Kiss
    Publication
    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    myc-PDGFRA-K627R (PDGFRA Synthetic, Human)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-K627R
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172597-plasmid-map-sequence-id-338908

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  19. pME-mCherry-PA-Rac1

    Plasmid
    #169042
    Purpose
    Middle entry vector for Gateway cloning that contains an mCherry fused with an optimized photoactivatable Rac1 gene insert
    Depositor
    Paola Arlotta
    Publication
    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d
    Insert
    PA-Rac-1 (RAC1 Human, Avena sativa (oat))
    Use
    Gateway entry cloning vector
    Tags
    6X His and Fused with mCherry
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    169042-plasmid-map-sequence-id-341784

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  20. pTM-RBDv2

    Plasmid
    #162785
    Purpose
    SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells
    Depositor
    Ivan Vorobiev
    Publication
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Insert
    SARS-CoV-2 S protein receptor binding domain (RBD) (S Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    Tags
    10xHis, c-myc, and hTPA leader
    Expression
    Mammalian
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162785-plasmid-map-sequence-id-333799

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Showing: 9841 - 9860 of 14665 results