We narrowed to 26,401 results for: Vit

1. Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP

   Plasmid

   #170327

   Purpose

   Double-floxed inverse Orientation HA and IRES-YFP tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. FRB-GFP-Gab1(Y628F/Y660F)

   Plasmid

   #188658

   Purpose

   FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe

   Depositor

   Andrei Karginov

   Article

   Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   GRB associated binding protein 1 (Gab1 Mouse)
   

   Tags

   FRB and GFP

   Expression

   Mammalian

   Mutation

   Y628F and Y660F

   Promoter

   CMV

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. GPR123-DuET

   Plasmid

   #213262

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR123 (ADGRA1 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLAP-BUBR1

   Plasmid

   #114052

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   BUBR1 (BUB1B Human)
   

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance)

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFPN1-N-gamma-tubulin (334-449)

   Plasmid

   #87859

   Purpose

   Fluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   gamma-tubulin 1 (TUBG1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   fragment from aa 334 to 449

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNOC_hfnCas12a-Nlux

   Plasmid

   #176239

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux under the control of Ribi promoter

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Promoter

   Ribosomal subunit

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-CMV-RPS4X-3xHA plasmid

   Plasmid

   #192246

   Purpose

   overexpression vector of RPS4X-3XHA

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   RPS4X CDS without stop coden (RPS4X Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-hSyn-Galpha-s ONE-GO

   Plasmid

   #214371

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   Galpha-s ONE-GO (GNAS Human)
   

   Use

   Lentiviral

   Promoter

   hSyn

   Available Since

   Feb. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pGAL4-DBD-FOXA3_AroLITE

   Plasmid

   #215640

   Purpose

   For overexpression of Gal4-DBD FOXA3 AroLITE

   Depositor

   Denes Hnisz

   Article

   Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

   Insert

   FOXA3 AroLITE (FOXA3 Human)
   

   Expression

   Mammalian

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGAL4-DBD-FOXA3_AroPERFECT

    Plasmid

    #215641

    Purpose

    For overexpression of Gal4-DBD FOXA3 AroPERFECT

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    FOXA3 AroPERFECT (FOXA3 Human)
    

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21a-C2m2-mKate

    Plasmid

    #208765

    Purpose

    To express C2m2-mKate in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-mKate (Mfge8 Mouse)
    

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    T7 promoter

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. GPR146-DuET

    Plasmid

    #213265

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR146 (GPR146 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PXPR007 sgCIC-2

    Plasmid

    #74959

    Purpose

    Cas9 + sgCIC-2 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    CIC (CIC Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-GRAB-rDA2mut

    Plasmid

    #208705

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) control sensor GRAB_rDA2mut in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) control sensor GRAB_rDA2mut
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. SECURE miniABEmax-V82G (pJUL1828)

    Plasmid

    #131313

    Purpose

    CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJM1-Flag-Rheb-4Rs

    Plasmid

    #165022

    Purpose

    expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)

    Depositor

    Ken Inoki

    Article

    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

    Insert

    Rheb-4Rs (RHEB Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    change lysine residues on position 109, 135, 151,…

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

    Plasmid

    #85111

    Purpose

    expression of recombinant human mature (delta19) ETFalpha wt without tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta19) ETFalpha (ETFA Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPBbsr2-Gnaq

    Plasmid

    #170298

    Purpose

    Encoding mouse Gnaq

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A recombinant mouse Gnaq (Gnaq Mouse)
    

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Asilent mutation was introduced into the sgRNA-ta…

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. FRB-GFP-Gab2(Y604F/Y634F)

    Plasmid

    #188659

    Purpose

    FRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to Phe

    Depositor

    Andrei Karginov

    Article

    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    GRB associated binding protein 2 (Gab2 Mouse)
    

    Tags

    FRB and GFP

    Expression

    Mammalian

    Mutation

    Y604F and Y634F

    Promoter

    CMV

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL4.23 A82V 2014 EBOV + Mucin-Like Domain

    Plasmid

    #86016

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Expression

    Mammalian

    Mutation

    Changed alanine 82 to valine

    Promoter

    CMV IE

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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