We narrowed to 12,202 results for: 112

1. pcDNA3-Rs4084-RBD-8h

   Plasmid

   #161825

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4084

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T321-D519)

   Promoter

   CMV

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Rs4231-RBD-8h

   Plasmid

   #161824

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4231

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T320-D518)

   Promoter

   CMV

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 3xFLAG TPP1 88-544 delta166-172

   Plasmid

   #53551

   Purpose

   mammalian expression of TPP1 full length with 166-172 replaced with "GSSG"

   Depositor

   Kathleen Collins

   Article

   Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

   Insert

   TPP1 (ACD Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   contains aa 88-544, with aa 166-172 replaced wit…

   Promoter

   CMV

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pHS1801

   Plasmid

   #205966

   Purpose

   JAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promoters

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas8-HNH proteins and CRISPR array
   

   Expression

   Bacterial

   Promoter

   Lac (proteins), pJ23119 (crRNA)

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS1232

   Plasmid

   #205969

   Purpose

   MWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promoters

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas5-HNH proteins and CRISPR array
   

   Expression

   Bacterial

   Promoter

   Lac (proteins), pJ23119 (crRNA)

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.24-Cd36-enhancer4

   Plasmid

   #138576

   Purpose

   Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17876439_17877752

   Depositor

   Lora Hooper

   Article

   Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

   Insert

   Cd36 Promoter (Cd36 Mouse)
   

   Use

   Luciferase

   Promoter

   Minimal promoter

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPN062

   Plasmid

   #91595

   Purpose

   Express sgRNA targeting human DFNA5

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   DFNA5 (GSDME Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPN063

   Plasmid

   #91596

   Purpose

   Express sgRNA targeting human DFNA5

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   DFNA5 (GSDME Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. NSP14-Flag

   Plasmid

   #210346

   Purpose

   Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUW-tetO-Meis1

    Plasmid

    #139824

    Purpose

    doxycycline-inducible expression of mouse Meis in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Meis1 (Meis1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR RPS27A K107/113R-3xFlag_IRES-GFP

    Plasmid

    #198392

    Purpose

    RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    ubiquitination site double mutant (K107/113R)

    Promoter

    EF1A

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO-RFP-IKZF3-sh2

    Plasmid

    #69043

    Purpose

    3rd generation lentiviral vector expressing IKZF3 shRNA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF3 (IKZF3 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-dLight1.2

    Plasmid

    #111069

    Purpose

    To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV1

    Insert

    dLight1.2
    

    Use

    AAV

    Tags

    Flag tag

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-BEACON1

    Plasmid

    #171697

    Purpose

    Expresses BEACON1 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

    Insert

    BEACON1
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_W104A/Y132D

    Promoter

    CMV

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. OR6K6_Deletion_gRNA2

    Plasmid

    #195196

    Purpose

    dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    OR6K6 dual gRNA (OR6K6 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. OR6K6_Deletion_gRNA1

    Plasmid

    #195195

    Purpose

    dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    OR6K6 dual gRNA (OR6K6 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. OR6K6_Deletion_gRNA3

    Plasmid

    #195197

    Purpose

    dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    OR6K6 dual gRNA (OR6K6 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-CERT-PH

    Plasmid

    #49572

    Purpose

    expresses the plekstrin homology domain of CERT/OSBP in mammalian cells

    Depositor

    Marci Scidmore

    Article

    Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

    Insert

    GPBP (CERT1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    expresses AA 1-122

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pGL4.24-Cd36-enhancer1

    Plasmid

    #138573

    Purpose

    Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17835541_17837355

    Depositor

    Lora Hooper

    Article

    Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

    Insert

    Cd36 enhancer (Cd36 Mouse)
    

    Use

    Luciferase

    Promoter

    Minimal promoter

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. plenti-UbcP-HA-YWAA-pGK-HYG

    Plasmid

    #107379

    Purpose

    Lentiviral expression of HA-YWAA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    CRBN-Y383A/W385A

    Promoter

    UbcP

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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