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pET26b(modified)-Lg-L

Plasmid

#216970

Purpose

Expression of Large BiT-Linker-GvpL

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpL

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-K-Lg

Plasmid

#216973

Purpose

Expression of GvpK-Linker-Large BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpK-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-Lg-U

Plasmid

#216980

Purpose

Expression of Large BiT-Linker-GvpU

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpU

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AP-SpyCatcher

Plasmid

#223636

Purpose

Protein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His10, AP fragment of split APEX2 tag

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher -AVI

Plasmid

#223635

Purpose

Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

AVI tag and His6

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGM84

Plasmid

#220172

Purpose

CRISRPi guide RNA against BTF3

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

BTF3 (sgRNA) (BTF3 Human)

Use

Lentiviral

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro CHKA_sg2

Plasmid

#218523

Purpose

sgRNA targeting human CHKA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CHKA (CHKA Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-SRRD-HA (in pLEX_307)

Plasmid

#214918

Purpose

constitutive expression of SRRD tagged with HA

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

HA

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk5a

Plasmid

#208988

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk5a (kcnk5a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMOSyt1C2AB(143-421)

Plasmid

#209007

Purpose

Recombinant protein expression of truncated syt1 construct

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 143-421 only

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8B4(E170Q)-HA

Plasmid

#209243

Purpose

Mammalian expression of ATP8B4 mutant E170Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B4 (ATP8B4 Human)

Tags

HA

Expression

Mammalian

Mutation

E170Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8B1-HA

Plasmid

#209226

Purpose

Mammalian expression of ATP8B1

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B1 (ATP8B1 Human)

Use

Retroviral

Tags

HA

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP11C(E184Q)-HA

Plasmid

#209263

Purpose

Mammalian expression of ATP11C mutant E184Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11C (ATP11C Human)

Use

Retroviral

Tags

HA

Mutation

E184Q

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8A1(E191Q)-HA

Plasmid

#209252

Purpose

Mammalian expression of ATP8A1 mutant E191Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8A1 (ATP8A1 Human)

Use

Retroviral

Tags

HA

Mutation

E191Q

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Atg20-24 pST39

Plasmid

#200439

Purpose

His6 tagged Atg20 and untagged Atg24 from S. cerevisiae for co-expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Inserts

Atg20
Atg24

Tags

6xHis-TEV

Expression

Bacterial

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-Mss4D636K-Kina

Plasmid

#202725

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:S. cerevisiae Mss4(377-756)(D636K) (MSS4 Budding Yeast)

Expression

Mammalian

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Mss4D636K-Kina

Plasmid

#202740

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:S. cerevisiae Mss4 (377-756)(D636K) (MSS4 Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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p2643

Plasmid

#198145

Purpose

Epitope tagging of effector protein SseF

Depositor

Michael Hensel

Article

Goser et al Nat Commun. 2023 Mar 4;14(1):1240. doi:

Insert

PsseA::sscB sseF::HA (sseF Salmonella enterica serovar Typhimurium)

Tags

HA

Expression

Bacterial

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2-del344-428_S

Plasmid

#147487

Purpose

Mammalian Expression of HsNot2-del344-428

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2-del344-428 (CNOT2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2_1-352_K

Plasmid

#146792

Purpose

Mammalian Expression of HsNot2_1-352

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2_1-352 (CNOT2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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