We narrowed to 24,950 results for: ina
-
Plasmid#153895PurposeBacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTRDepositorInsertfragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionBacterialMutationNearly native encoding;inactivating mutations in …PromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBa.GFP-KIF1Balpha tail 387-1150
Plasmid#134619PurposeMammalian expression of mouse KIF1Balpha 387-1150DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKIF1Balpha (Kif1b Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 386 N-terminal residues.PromoterChicken Beta actinAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_WW-PolyA
Plasmid#112290PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. WQDP (199-202) in the first WW domain was changed to AQDA, and the WLDP (258-261) of the second WW domain was changed to ALDADepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with mutations in the WW domain…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G
Plasmid#113953Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, I132S, S212N, and …PromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKAR1-NES
Plasmid#84632PurposeUnimolecular, FRET-based JNK activity reporter; cytosol targetedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertECFP-FHA1-JNKsubDD-Citrine-NES
Tags6xHis, Citrine, ECFP, Nuclear export signal (NES)…ExpressionMammalianPromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL1-G20TM
Plasmid#109019Purposestable lentiviral expression of cDNADepositorInsertATL1-deltaTM (ATL1 Human)
UseLentiviralTagseGFPExpressionMammalianMutationtruncated form without C-terminal ER transmembran…PromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
B270 + SMARCAL1 sgSTOP
Plasmid#100717PurposeB270 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgSTOP targeting SMARCAL1 (cloned using BbsI) and ATP1A1 (SMARCAL1 Human)
UseCRISPRExpressionMammalianPromoterU6 promotersAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP3-44
Plasmid#91780PurposeMammalian expression of low affinity calcium-sensing fluorescent proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCaMP3-44
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTE4566
Plasmid#88904PurposeExpresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nucleaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsMb crRNA
hMbCpf1(D986A)
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMV and human U6Available sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 8 GFP RFP
Plasmid#83564PurposeExpresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.DepositorInsertPKC alpha (PRKCA Human)
Tags10 nm ER/K Helix, FLAG purification tag, TEV Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Jam1-Fc-His
Plasmid#72078PurposeExpresses the extracellular region of the JAM-1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIS1 IL6R
Plasmid#60796PurposeLuciferase reporter to measure miR-155 mediated differential repression. Contains IL6R 3' UTR and wild-type miR-155 sitesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIL6R 3'UTR and wild-type miR-155 binding site (IL6R Human)
UseLuciferaseExpressionMammalianPromoterthymidine kinase (HSV-TK promoter)Available sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-MAPTau-N-10
Plasmid#55498PurposeLocalization: Microtubules, Excitation: 462, Emission: 492DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMAPTau (MAPT Human)
TagsmTFP1ExpressionMammalianPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSP9-bio
Plasmid#47740PurposeExpresses enzymatically monobiotinylated full-length MSP9 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorPublicationInsertCodon-optimised MSP9
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
CLAG3.2-bio
Plasmid#47797PurposeExpresses enzymatically monobiotinylated full-length CLAG3.2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorPublicationInsertCodon-optimised CLAG3.2
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
RhopH2-bio
Plasmid#47798PurposeExpresses enzymatically monobiotinylated full-length RhopH2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorPublicationInsertCodon-optimised RhopH2
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
PLK3 335-646-pEGFP
Plasmid#23267DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPLK3 (PLK3 Human)
TagspEGFPExpressionMammalianMutationaa 335-646Available sinceDec. 20, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
426Gal-FUS-1-501aa-YFP
Plasmid#29601DepositorAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
426Gal-FUS-1-373aa-YFP
Plasmid#29598DepositorAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
p2Flag CMV2-ZO-2-N
Plasmid#27418DepositorPublicationInsertZonula Occludens 2 (TJP2 Human)
Tags2xFlagExpressionMammalianMutationDeletion of the carboxy-terminus of ZO-2, so only…Available sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only