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pvimentin-miRFP713

Plasmid

#136569

Purpose

Vimentin labeling with near-infrared fluorescent protein miRFP713

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

vimentin-miRFP713

Tags

miRFP713

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pUCBB-ctH6-eGFP

Plasmid

#32562

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Promoter

Consitutive lac

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR221-CAG.Pro

Plasmid

#79490

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) CAG promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CAG Promoter

Use

Multisite gateway entry clone

Promoter

CAG Promoter

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pET11d_Rev

Plasmid

#119322

Purpose

Expression of protein Rev from HIV

Depositor

Francisco Blanco, Robert Tycko

Article

Blanco et al J Mol Biol. 2001 Nov 2;313(4):845-59. do

Insert

Rev (rev HIV B1)

Expression

Bacterial

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSC6

Plasmid

#104780

Purpose

pSC218UG-At Ubiquitin10:Gateway expression cassette binary vector

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAd-Track SIRT1

Plasmid

#8440

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Expression

Mammalian

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pMOD_C3010

Plasmid

#91098

Purpose

Module C, Promoter: ZmUbi, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

ZmUbi

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0902

Plasmid

#91026

Purpose

Module A, Promoter: AtUbi10, Gene: TREX2-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TREX2-P2A-AtCas9

Use

CRISPR

Promoter

AtUbi10

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shACO1

Plasmid

#169884

Purpose

Suppress ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

shRNA against ACO1

Use

Lentiviral and RNAi

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pJG80

Plasmid

#89282

Purpose

Entry vector containing TaCas9-nos terminator between attL1 and attR5 sites

Depositor

Daniel Voytas

Article

Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

Insert

TaCas9

Use

CRISPR

Expression

Plant

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pPRIME-dsRed-shvgat

Plasmid

#71385

Purpose

Expresses dsRed and shRNA against vgat under the control of the CMV promoter in eukaryotic cells

Depositor

William Wisden

Article

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

CMV promoter-dsRed-mir30-shvgat

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

CMV enhancer/promoter

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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ER2-hfYFP

Plasmid

#186518

Purpose

Localization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pT7C-HSPA1L

Plasmid

#177660

Purpose

Recombinant protein synthesis of human HSPA1L

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

HSPA1L (HSPA1L Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE:CloverCP-EFS:ZeoR

Plasmid

#68478

Purpose

Lentivirus for establishment of doxycycline-inducible cell line expression CloverCP

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

CloverCP
Zeocin Resistance

Use

Lentiviral

Tags

"CP" degradation domain

Expression

Mammalian

Promoter

EFS and pTRE

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMP4518

Plasmid

#107778

Purpose

Expression of EYFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EYFP in pBBR1-mcs5

Tags

pLacZ-EYFP

Expression

Bacterial

Promoter

pLacZ

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-shTACC3

Plasmid

#59355

Purpose

Knocks down TACC3 in mammalian cells via shRNA and co-expresses GFP.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

EGFP

Use

RNAi

Expression

Mammalian

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pAV0751

Plasmid

#133485

Purpose

Expression in fission yeast from promoter pnmt1 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(nmt1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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1113 pSG5L HA PTEN D3

Plasmid

#10752

Depositor

William Sellers

Article

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

D3: S380D, T382D, T383D

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pCLX-M38s/TOP-dGFP

Plasmid

#114295

Purpose

Expression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoter

Depositor

Patrick Salmon

Article

Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

Insert

dGFP

Use

Lentiviral

Mutation

destabilized GFP

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp5

Plasmid

#163589

Purpose

Expresses BMP-5 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp5 (Bmp5 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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