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FusionRed-PlastinC-10

Plasmid

#56130

Purpose

Localization: Cytoskeleton, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Plastin (PLS1 Human)

Use

Tags

FusionRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pGK:HygroR-CMV:mKO2-C_UTR

Plasmid

#68481

Purpose

Lentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mKO2-C_UTR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

shRNA sensor cassette "B" added to 3&#x…

Promoter

CMV and pGK

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pSC44

Plasmid

#104818

Purpose

CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from AtUBQ10 promoter.

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Insert

Glyma.04g057400

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRQ-Id1HA

Plasmid

#18112

Depositor

Alex Swarbrick

Article

Swarbrick et al Proc Natl Acad Sci U S A. 2008 Apr 8. 10

Insert

Inhibitor of differentiation 1 (Id1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pmalc4x_I499F_TEV

Plasmid

#47703

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499F

Depositor

Raquel Lieberman

Article

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Olfactomedin-like domain with I499F mutation (MYOC Human)

Use

Tags

MBP

Expression

Bacterial

Mutation

changed I499F

Promoter

Ptac

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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MEIS1A-pSP65

Plasmid

#21014

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1A (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Expression

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pLL3.7 shRNA against CRTC1

Plasmid

#72710

Purpose

Lentiviral expression of shRNA against CRTC1 (3rd generation)

Depositor

Freddy Jeanneteau

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

CRTC1

Use

Lentiviral

Tags

CMV-EGFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pMSCV FHA-ATG12

Plasmid

#27049

Depositor

Jayanta Debnath

Article

Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Insert

autophagy-related 12 (yeast) (Atg12 Mouse)

Use

Retroviral

Tags

HA and flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-flex-rev-g2-2A-Venus

Plasmid

#71410

Purpose

This plasmid is pAAV-panpromoter-flex-rev-gamma2F77-2A-Venus. Confers zolpidem sensitivity on zolpidem-insensitive neurons.

Depositor

William Wisden

Article

Uygun et al J Neurosci. 2016 Nov 2;36(44):11171-1118

Inserts

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

mouse hdc gene promoter, works as a pan neuronal …

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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FN207

Plasmid

#50497

Depositor

Kenneth Yamada

Article

Danen et al J Biol Chem. 1995 Sep 15;270(37):21612-8

Insert

NM_001306129.1 (FN1 Human)

Use

Tags

Expression

Bacterial

Mutation

repeats 6-10

Promoter

T7

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
px459-H3f3a KO sgRNA

Plasmid

#186940

Purpose

H3f3a KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

H3f3a KO sgRNA (H3f3a Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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px459-H3f3b KO sgRNA

Plasmid

#186941

Purpose

H3f3b KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

H3f3b KO sgRNA (H3f3b Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_A0608

Plasmid

#91018

Purpose

Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_D10A nickase

Use

CRISPR

Tags

Expression

Mutation

D10A

Promoter

EC1.2

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-MBP-(CBX1)3-eReader

Plasmid

#135090

Purpose

Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

Depositor

Marcey Waters

Article

Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

Insert

CBX1 chromobox protein 1, engineered x3

Use

Tags

6xhis-tag and MBP

Expression

Bacterial

Mutation

lysine 43 mutated to alanine; aspartic acid 59 mu…

Promoter

T7

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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HpMscS

Plasmid

#78310

Purpose

H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag

Depositor

Douglas Rees

Article

Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:

Insert

Mechanosensitive channel of small conductance

Use

Tags

6x His Tag and FLAG Tag

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD_A1710

Plasmid

#91039

Purpose

Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-TaCas9_H840A nickase

Use

CRISPR

Tags

Expression

Mutation

H840A

Promoter

ZmUbi

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-miRE_shBRD9_508-SV40-GFP

Plasmid

#75138

Purpose

Retroviral expression plasmid encoding shBRD9_508 (targeting human BRD)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBRD9_508

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C49,50,83,103A

Plasmid

#58421

Purpose

Expresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Cysteines 49, 50, 83, and 103 to Alanine

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pMIEG3-NFAT2

Plasmid

#40351

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2006 Jan 15;176(2):880-8.

Insert

NFAT2 (NFATC1 Mouse)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pMIR-D10 mutUTR

Plasmid

#19116

Depositor

Bob Weinberg

Article

Ma et al Nature. 2007 Oct 11. 449(7163):682-8.

Insert

HOXD10 mut 3' UTR (HOXD10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only