We narrowed to 26,401 results for: Vit

1. pDEST_FLAG-HUWE1_UBA/UIM/ UBM-mut

   Plasmid

   #187154

   Purpose

   mammalian cell expression of FLAG-HUWE1 L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PFKFB3 gRNA (BRDN0001147151)

   Plasmid

   #76461

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PFKFB3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PFKFB3 (PFKFB3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PFKFB3 gRNA (BRDN0001147212)

   Plasmid

   #76463

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PFKFB3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PFKFB3 (PFKFB3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCALNL-mutKir2.1

   Plasmid

   #125579

   Purpose

   Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   Kir2.1 (Kcnj2 Mouse)
   

   Use

   Cre/Lox

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   A non-conducting mutant (GYG -> AAA).

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. STK4 gRNA (BRDN0001149002)

   Plasmid

   #76074

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human STK4

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   STK4 (STK4 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-SYN_mEGFP-CREB (S133A)

   Plasmid

   #137010

   Purpose

   AAV backbone, mEGFP-CREB donor for G-CREB with S133A mutation

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

   Insert

   CREB1 (Creb1 Rat)
   

   Use

   AAV

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Changes Serine 133 into Alanine

   Promoter

   hSynapsin

   Available Since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pF712-pET28a-Hs-delta44-GCDH-NHis wt

   Plasmid

   #85113

   Purpose

   expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta44) GCDH (GCDH Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   silent SNP: G1041T

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. HTR4-DuET

   Plasmid

   #213324

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   HTR4 (HTR4 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-FLIP pr5

   Plasmid

   #19130

   Depositor

   Wafik El-Deiry

   Article

   Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

   Insert

   FLIP promoter (CFLAR Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   The sequence corresponds to positions -503 to +28…

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. PIKFYVE gRNA (BRDN0001144909)

    Plasmid

    #76890

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIKFYVE

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIKFYVE (PIKFYVE Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PRKAA1 gRNA (BRDN0001146526)

    Plasmid

    #76253

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAA1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAA1 (PRKAA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EGFP.T2A.NCLX

    Plasmid

    #181873

    Purpose

    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Inserts

    solute carrier family 8 member B1 (Slc8a1 Mouse)
    EGFP
    

    Use

    AAV and Adenoviral

    Tags

    HA and myc

    Expression

    Mammalian

    Promoter

    synthetic hybrid CAG promoter and synthetic hybri…

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. MCHR1-DuET

    Plasmid

    #213337

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MCHR1 (MCHR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. DN1-Gli3 in AINGpCAGGS

    Plasmid

    #121970

    Purpose

    medium Gli3 transcriptional activator

    Depositor

    James Briscoe

    Article

    Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

    Insert

    DN2Gli3 (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc

    Expression

    Mammalian

    Mutation

    N terminus deletion (nt1-864)

    Available Since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGAL4-DBD-CEBPa-WT

    Plasmid

    #215601

    Purpose

    For overexpression of Gal4-DBD CEBPa WT

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    CEBPa (CEBPA Human)
    

    Expression

    Mammalian

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1 shETV1-A

    Plasmid

    #74973

    Purpose

    shETV1 shRNA

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV1 (ETV1 Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PIP5K1C gRNA (BRDN0001148198)

    Plasmid

    #77665

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP5K1C

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP5K1C (PIP5K1C Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. PIP5K1C gRNA (BRDN0001145736)

    Plasmid

    #77666

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP5K1C

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP5K1C (PIP5K1C Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PIKFYVE gRNA (BRDN0001148075)

    Plasmid

    #76891

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIKFYVE

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIKFYVE (PIKFYVE Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hAID-BE3 (pRZ352)

    Plasmid

    #131316

    Purpose

    CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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