We narrowed to 145 results for: 2a peptide
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Plasmid#198223PurposeLentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFPDepositorInsertPAG1 (PAG1 Human)
UseLentiviralTagsFollowed by EGFP, after a flexible linker and Pre…ExpressionMammalianMutationNoneAvailable SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCWXPG-UBI-Ngn3PDX1MafA
Plasmid#114303PurposeConstitutive expression of Ngn3, PDX1 and MafA proteins from a precursor with self-cleavable 2A peptidesDepositorInsertNgn3 (NEUROG3 Human)
UseLentiviralMutationNgn3, PDX1 and MafA proteins are expressed from a…Available SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX
Plasmid#52421PurposeExpresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherryDepositorInsertFusion NLS-EGFP-P2A-Cherry-CAAX
ExpressionMammalianPromoterCMVAvailable SinceApril 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro
Plasmid#69546PurposeTet-On system expressing MyoD-2A-GFP-2xNLS.DepositorInsertsUseLentiviralTags2x nuclear localization sequence and T2A "sk…MutationRemoved stop codon to fuse it to the 2A-eGFPnls i…Available SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-TLCV2
Plasmid#196331Purposethe TLCV2 plasmid with Tol2 recombination sites for integrationDepositorInsertCas9-2A-eGFP
UseCRISPR; Fish expressionExpressionBacterial, Mammalian, and Y…PromoterTight TRE promoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cre Shine
Plasmid#37404PurposeThis is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.DepositorInsertCre Shine
UseCre/LoxTagseGFPExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pR I-Ab beta Derp1-117
Plasmid#113639PurposeInsect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptideDepositorInsertI-Ab beta Derp1-117 (H2-Ab1 Mouse)
Tags6x His tag, fused to Der p 1 (117-127) peptide vi…ExpressionInsectMutationtransmembrane and cytoplasmic domains truncatedPromoterMetallothioneinAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
tetO-ALN
Plasmid#43918DepositorUseLentiviral; Doxycycline inducibleTags6xHis and V5ExpressionMammalianMutationGylcine-serine-glycine (GSG) linker followed by v…PromoterTRE promoter, Tet-ONAvailable SinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAN012
Plasmid#220051PurposeReporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide (positive control).DepositorInsertGFP-fLuc
ExpressionMammalianAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKP332 (Lenti-OSK1)
Plasmid#21627DepositorUseCre/Lox and LentiviralExpressionMammalianAvailable SinceNov. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pME-GFP-P2A
Plasmid#80807PurposeGFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptideDepositorInsertGFP-P2A
Available SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAN013
Plasmid#220050PurposeReporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide. A stop codon is inserted at the 3'-end of the gfp gene, which permits GFP, but not luciferase expression.DepositorInsertGFP-stop-fLuc
ExpressionMammalianAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dimer cleaved
Plasmid#198199PurposeFRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavageDepositorInsertmVenus-T2A-mTurquoise2
ExpressionMammalianMutationA206K bothAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
786 pCSmAKT2Acherry
Plasmid#31167DepositorInsertAkt
UseExpression vectorTagsMyr and mCherryMutationmembrane tethered AktAvailable SinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTS1018-Tier1-PhCMV-p2A
Plasmid#169491PurposeTier-1 vector encoding a self-cleaving peptides p2a sequence (PhCMV-p2A-pA).DepositorInsertPCMV-driven 2A sequence of porcine teschovirus-1
ExpressionMammalianPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAH-POLY-eRF1(E55D)
Plasmid#157925PurposeEncodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cellsDepositorInsertPOLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
ExpressionMammalianPromoterCMVAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pP2A-mCherry-N1
Plasmid#84329PurposeEncodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherryDepositorInsertP2A-mCherry
ExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR
Plasmid#126705Purposedonor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequenceDepositorInsertGFP
UseCRISPRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
mE-mCherry2A
Plasmid#236248PurposeMiddle entry fluorescent mCherry reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separatelyDepositorInsertmCherry2A
ExpressionMammalianMutationAdded a viral2A sequence to mCherry with no stop …Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ121
Plasmid#99325PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequenceDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENExpressionPlantAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only