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pGAL4-DBD-D6Y_AroPERFECT

Plasmid

#215634

Purpose

For overexpression of Gal4-DBD D6Y AroPERFECT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

D6Y AroPERFECT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-S6Y_AroPERFECT

Plasmid

#215631

Purpose

For overexpression of Gal4-DBD S6Y AroPERFECT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

S6Y AroPERFECT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-PDX1_AroPERFECT

Plasmid

#215644

Purpose

For overexpression of Gal4-DBD PDX1 AroPERFECT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

PDX1 AroPERFECT (PDX1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-mEGFP-hCEBPa_AroPERFECT_IS15

Plasmid

#215615

Purpose

For integration of CEBPa AroPERFECT IS15 mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroPERFECT IS15 (CEBPA Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-mEGFP-hCEBPa_AroPERFECT_IS10

Plasmid

#215616

Purpose

For integration of CEBPa AroPERFECT IS10 mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroPERFECT IS10 (CEBPA Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGal4-DBD-OCT4_AroPERFECT_C

Plasmid

#215625

Purpose

For overexpression of Gal4-DBD OCT4 AroPERFECT C

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

OCT4 AroPERFECT C

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGal4-DBD-OCT4_AroPERFECT_N

Plasmid

#215624

Purpose

For overexpression of Gal4-DBD OCT4 AroPERFECT N

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

OCT4 AroPERFECT N

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 3xSA

Plasmid

#195207

Purpose

Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphorylation-deficient mutant (S162/339/340A)

Depositor

David Bryant, Anthony Bretscher

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

S162/339/340A

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 3xSD

Plasmid

#195208

Purpose

Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphomimetic mutant (S162/339/340D)

Depositor

David Bryant, Anthony Bretscher

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

S162/339/340D

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 F355R

Plasmid

#195209

Purpose

Mammalian expression vector for GFP tagged NHERF1. Ezrin interaction-deficient mutant

Depositor

David Bryant, Anthony Bretscher

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

F355R

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 PDZ1*

Plasmid

#195210

Purpose

Mammalian expression vector for GFP tagged NHERF1. PDZ1 GYGF>GYAA motif mutant

Depositor

David Bryant, Anthony Bretscher

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

PDZ1 GYGF>GYAA

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 PDZ1/2*

Plasmid

#195211

Purpose

Mammalian expression vector for GFP tagged NHERF1. Dual PDZ1/2 GYGF>GYAA motif mutant

Depositor

David Bryant, Anthony Bretscher

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Dual PDZ1/2 GYGF>GYAA motif mutant

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 PDZ2*

Plasmid

#195212

Purpose

Mammalian expression vector for GFP tagged NHERF1. PDZ2 GYGF>GYAA motif mutant

Depositor

David Bryant, Anthony Bretscher

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

PDZ2 GYGF>GYAA

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB9358(VII-1_Markerfree_BackBone)

Plasmid

#161599

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VII-1, (Chr VII: 438509..438490)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9359(VIII-1_Markerfree_BackBone)

Plasmid

#161600

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VIII-1, (Chr VIII: 293615..293634)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB9361(XIII-1_Markerfree_BackBone)

Plasmid

#161602

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XIII-1, (Chr XIII: 306804..306823)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB9363(XVI-1_Markerfree_BackBone)

Plasmid

#161604

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XVI-1, (Chr XVI: 406267..406286)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAT15541_dCBE-SuperFi-Cas9

Plasmid

#184371

Purpose

Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

dCBE-SuperFi-Cas9

Use

CRISPR

Tags

3X FLAG, SV40 NLS, and UGI

Expression

Mammalian

Mutation

D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

Promoter

EF-1α core promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15543_dABE-SuperFi-Cas9

Plasmid

#184373

Purpose

Mammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

dABE-SuperFi-Cas9

Use

CRISPR

Tags

FLAG, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

Promoter

EF-1α core promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAT15545_dABE8e-SuperFi-Cas9

Plasmid

#184375

Purpose

Mammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

dABE8e-SuperFi-Cas9

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

Promoter

EF-1α core promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge