We narrowed to 9,122 results for: Pol

1. HO-Poly-KanMX4-HO

   Plasmid

   #51662

   Purpose

   yeast plasmid for integration of target sequence at HO locus, Kan selection

   Depositor

   David Stillman

   Article

   Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

   Type

   Empty backbone

   Use

   Yeast integration

   Expression

   Yeast

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE30-hPolB

   Plasmid

   #70761

   Purpose

   bacterial expression of human DNA Polymerase beta (POLB)

   Depositor

   Primo Schaer

   Article

   Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

   Insert

   POLB (POLB Human)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   R333L

   Promoter

   T5/lac operon

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9-polyA

   Plasmid

   #72602

   Purpose

   express hCas9 byT7 promoter with polyadenine tails

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

   Insert

   Cas9-polyadenine
   

   Tags

   81-bp polyadenylation

   Promoter

   T7

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOpen-SP6rpol

   Plasmid

   #165516

   Purpose

   DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   SP6 RNA Polymerase
   

   Use

   Synthetic Biology

   Available Since

   July 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. HDR-HaloPOLQ

   Plasmid

   #211637

   Purpose

   halo donor

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   Puro-LoxHaloTag
   

   Tags

   flag

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBluescript II SK--POLQ

   Plasmid

   #64873

   Purpose

   cloning vector for human POLQ

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Insert

   POLQ (POLQ Human)
   

   Expression

   Bacterial

   Available Since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAH-POLY

   Plasmid

   #64915

   Purpose

   Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

   Depositor

   Huiwang Ai

   Article

   Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

   Inserts

   Polyspecific aminoacyl-tRNA Synthetase
   Amber Suppression tRNA
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CMV and U6/H1

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEP PolI

   Plasmid

   #11722

   Depositor

   Lawrence Loeb

   Article

   Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

   Insert

   DNA polymerase I EP (polA E. coli)
   

   Expression

   Bacterial

   Mutation

   D424A, I709N, A759R. Error-prone version of PolI.

   Available Since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWT PolI

   Plasmid

   #11721

   Depositor

   Lawrence Loeb

   Article

   Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

   Insert

   DNA polymerase I (polA E. coli)
   

   Expression

   Bacterial

   Mutation

   wild-type

   Available Since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOpen-KTHEAQpol

    Plasmid

    #165542

    Purpose

    Exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    KlenTaq1 polymerase
    

    Use

    Synthetic Biology

    Available Since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. mCherry-POLE1

    Plasmid

    #215140

    Purpose

    Expresses mCherry-POLE1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    POLE1 (POLE Human)
    

    Use

    Retroviral

    Tags

    mCherry_FWR

    Expression

    Mammalian

    Mutation

    Silent mutations in amino acid 80-86 and 211-217 …

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOpen-Pwopol

    Plasmid

    #165562

    Purpose

    DNA polymerase; additionally exhibits 3' to 5' exonuclease activity.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    Pwo DNA Polymerase
    

    Use

    Synthetic Biology

    Available Since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. POLG-pcDNA3.1

    Plasmid

    #191487

    Purpose

    Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.

    Depositor

    Hans Zempel

    Insert

    POLG (POLG Human)
    

    Tags

    V5 tag, 6X His

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. EP-Pol vector

    Plasmid

    #131686

    Purpose

    A lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymerase

    Depositor

    Matthew D. Shoulders

    Article

    Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

    Insert

    Adenovirus DNA polymerase
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    F421Y, D827A

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOpen-K12polLF

    Plasmid

    #165511

    Purpose

    DNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    DNA Polymerase I, Large (Klenow) Fragment
    

    Use

    Synthetic Biology

    Available Since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pOpen-BsupolLF

    Plasmid

    #165563

    Purpose

    Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    Bsu DNA Polymerase I, Large Fragment
    

    Use

    Synthetic Biology

    Available Since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pOpen-THEAQpolA

    Plasmid

    #165512

    Purpose

    DNA polymerase used in routine PCR method because of its high thermostability, standard testing, screening and output testing.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    Taq DNA Polymerase
    

    Use

    Synthetic Biology

    Available Since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. FLAG-Pol-II 1-22

    Plasmid

    #35178

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal domain repeats 23-52

    Promoter

    CMV

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

19. FLAG-Pol-II 31-52

    Plasmid

    #35179

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal domain repeats 1-30

    Promoter

    CMV

    Available Since

    Oct. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pOpen-9N7polA

    Plasmid

    #165500

    Purpose

    Thermophilic DNA polymerase; genetically engineered to have a decreased 3'-5' proofreading exonuclease activity

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    9 degrees N-7 DNA polymerase
    

    Use

    Synthetic Biology

    Available Since

    July 26, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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