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pXPA-fapO-RgTALsyn

Plasmid

#100946

Purpose

Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

GAP (Constitutive)

Availability

Academic Institutions and Nonprofits only

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pDONR_P4-P1R_5'arm_apobb.1

Plasmid

#128662

Purpose

apobb.1 homology arm 5' gateway clone

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

Genomic sequence upstream of apobb.1 stop codon (apobb.1 Zebrafish)

Use

Multi-step gateway cloning

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pDONR_P2R-P3_3'arm_apobb.1

Plasmid

#128681

Purpose

apobb.1 homology arm 3' gateway clone

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

apobb.1 (apobb.1 Zebrafish)

Use

Multi-step gateway cloning

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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rat APOBEC1-EGFP

Plasmid

#112857

Purpose

plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein

Depositor

Silvestro Conticello

Article

Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

Insert

apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Apobec1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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rat APOBEC1 E63A

Plasmid

#112861

Purpose

plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

APOBEC1 (Apobec1 Rat)

Use

Tags

Expression

Mammalian

Mutation

changed Glutamic acid 63 to Alanine

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-IVa

Plasmid

#121902

Purpose

Plasmid for making zebrafish apoA-IVa in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoA4a (apoa4a Zebrafish)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-IVb.1

Plasmid

#121903

Purpose

Plasmid for making zebrafish apoA-IVb.1 in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

Apoa4b.1 (apoa4b.1 Zebrafish)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-IVb.2

Plasmid

#121904

Purpose

Plasmid for making zebrafish apoA-IVb.2 in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

Apoa4b.2 (apoa4b.2 Zebrafish)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-IVb.3

Plasmid

#121905

Purpose

Plasmid for making zebrafish apoA-IVb.3 in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

apoa4b.3 (apoa4b.3 Zebrafish)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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TLCV2-APOBEC1-YTH

Plasmid

#178949

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTH-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Mutation

Promoter

tight TRE

Availability

Academic Institutions and Nonprofits only

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TLCV2-APOBEC1-YTHmut

Plasmid

#178950

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTHmut-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Mutation

Promoter

tight TRE

Availability

Academic Institutions and Nonprofits only

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pCMV_APOBEC1-UdgX-nCas9

Plasmid

#163531

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

APOBEC1-UdgX-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)

Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV_APOBEC1-UdgX-NG-nCas9

Plasmid

#163533

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

APOBEC1-UdgX-NG-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4-AmAPOBEC1

Plasmid

#138342

Purpose

Mammalian expression plasmid for BE4-AmABOBEC1

Depositor

Nicole Gaudelli

Article

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Insert

BE4-AmAPOBEC1

Use

Synthetic Biology

Tags

BP-NLS

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4-SsAPOBEC3B

Plasmid

#138343

Purpose

Mammalian expression plasmid for BE4-SsAPOBEC3B

Depositor

Nicole Gaudelli

Article

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Insert

BE4-SsAPOBEC3B

Use

Synthetic Biology

Tags

BP-NLS

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4-PpAPOBEC1

Plasmid

#138349

Purpose

Mammalian expression plasmid for BE4-PpABOBEC1

Depositor

Nicole Gaudelli

Article

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Insert

BE4-PpAPOBEC1

Use

Tags

BP-NLS

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-APOBEC1-YTH

Plasmid

#131636

Purpose

Expresses APOBEC1 fused to the YTH domain

Depositor

Kate Meyer

Article

Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

Insert

APOBEC1 fused to YTH domain (YTHDF2 Rat, Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV-APOBEC1-YTHmut

Plasmid

#131637

Purpose

Expresses APOBEC1 fused to a mutant YTH domain with diminished m6A binding

Depositor

Kate Meyer

Article

Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

Insert

APOBEC1-YTHmut-HA (YTHDF2 Rat, Human)

Use

Tags

HA

Expression

Mammalian

Mutation

YTH domain lacks AA 385-409 comprising the m6A-bi…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pUC19-hAPOE-R-loop

Plasmid

#134901

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

APOE promoter (APOE Human)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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hsp70l: hAPOB48-mCherry.

Plasmid

#140639

Purpose

Heat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.

Depositor

Steven Farber

Article

Walsh et al J Lipid Res. 2019 Dec 30. pii: jlr.RA119

Inserts

hsp70l (hsp70l Zebrafish)
APOB (APOB Human)

Use

Zebrafish expression

Tags

mCherry

Expression

Mutation

APOB48: amino acid 1-2180

Promoter

hsp70l

Availability

Academic Institutions and Nonprofits only

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