Skip to main content
Addgene
Showing: 81 - 100 of 1126 results

  9. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

    Plasmid
    #105805
    Purpose
    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-Cerulean; ORF2: TEV-Venus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105805-plasmid-map-sequence-id-207906

    Enlarge

  17. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid
    #105806
    Purpose
    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-Cerulean; ORF2: TEV-Amber
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105806-plasmid-map-sequence-id-207907

    Enlarge

  18. pET15b/EYFP-GgVcl 884-1066-ECFP FRET

    Plasmid
    #46179
    Depositor
    Susan Craig
    Insert
    Vcl 884-1066 (VCL Chicken)
    Use
    Tags
    ECFP, EYFP, and His
    Expression
    Bacterial
    Mutation
    aa 884-1066 (Vt, tail domain)
    Promoter
    T7
    Available since
    July 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    46179-plasmid-map-sequence-id-198213

    Enlarge

  20. α-catenin ECFP-YPET FRET ΔF-actin binding site

    Plasmid
    #198245
    Purpose
    Alpha catenin FRET conformation sensor protein with C-terminal truncation removing ability to bind F-actin.
    Depositor
    Deborah Leckband
    Article
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Insert
    α-catenin ECFP-YPET FRET sensor ΔF-actin binding site
    Use
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted F-actin binding site on a-catenin (849-90…
    Promoter
    CMV
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    198245-plasmid-map-sequence-id-401145

    Enlarge

Showing: 81 - 100 of 1126 results