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Showing: 81 - 100 of 228 results

  9. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Article
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73796-plasmid-map-sequence-id-237125

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  10. p416 1-F GW

    Plasmid
    #58543
    Purpose
    pRS416 carrying Gateway cassette for a single insert
    Depositor
    Leonid Kruglyak
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    58543-plasmid-map-sequence-id-90631

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  11. p416 3-F GW

    Plasmid
    #58552
    Purpose
    pRS416 carrying Gateway cassette for three inserts
    Depositor
    Leonid Kruglyak
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    58552-plasmid-map-sequence-id-90656

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  13. ampl43

    Plasmid
    #161830
    Purpose
    pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.
    Depositor
    Saeed Tavazoie
    Article
    Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    161830-plasmid-map-sequence-id-318315

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  17. pAV116

    Plasmid
    #63183
    Purpose
    pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63183-plasmid-map-sequence-id-104859

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  18. pAV11K

    Plasmid
    #63184
    Purpose
    pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63184-plasmid-map-sequence-id-104886

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  19. p41Hyg 1-F GW

    Plasmid
    #58547
    Purpose
    CEN vector with Hygromycin resistance marker for single Gateway insertion
    Depositor
    Leonid Kruglyak
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    58547-plasmid-map-sequence-id-90634

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  20. p41Hyg 3-F GW

    Plasmid
    #58555
    Purpose
    CEN vector with Hygromycin resistance marker for triple Gateway insertion
    Depositor
    Leonid Kruglyak
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    58555-plasmid-map-sequence-id-268576

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Showing: 81 - 100 of 228 results