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Plasmid#47812PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD5 efficiency.DepositorInsertpromoter 14 and BCD5
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB801
Plasmid#47846PurposeBIOFAB reporter plasmid for measuring trp_att_L126 termination efficiencyDepositorInserttrp_att_L126 terminator
UseSynthetic BiologyTagsExpressionBacterialMutationLoop mutation in trp TerminatorPromoterpLTetO1Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3881
Plasmid#47843PurposeBIOFAB RFP reporter plasmid for measuring promoter 12 + BCD1 efficiency.DepositorInsertpromoter 12 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3905
Plasmid#47808PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD1 efficiency.DepositorInsertpromoter 14 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pFA12
Plasmid#46792PurposeTo express a version of Fun30 with a mutated CUE domain in budding yeastDepositorInsertFUN30, CUE domain mutated (FUN30 Budding Yeast)
UseTagsV5ExpressionYeastMutationFP82AAPromoterAvailable sinceAug. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-melHis10TEV
Plasmid#159428Purposeempty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sitesDepositorTypeEmpty backboneUseTagsmelittin signal sequence (removed in vivo by sign…ExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-M2
Plasmid#135584PurposeHigh eukaryotic dual protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorTagsExpressionInsectMutationPromoterpolyhedrin of AcMNPVAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18c
Plasmid#135555PurposeExpress mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.DepositorInsertMunc18c
UseTags6xHis tag and TEV siteExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG654 pFAST
Plasmid#172861PurposeExpresses pFAST in mammalian cellsDepositorInsertpFAST
UseTagsMyc tag (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a LEU2
Plasmid#61923PurposeExpression of K. lactis LEU2 cloned into pFA6aDepositorInsertLEU2
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1
Plasmid#41595DepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6
Plasmid#39296DepositorInsertKanR
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His10FLAG
Plasmid#157736Purposeempty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneUseTagsHis10 + 3xFLAG, enterokinase-cleavableExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFast-hRad1
Plasmid#222471PurposeExpresses hRad1 in insect cellsDepositorInserthRad1 (RAD1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFast-hHus1
Plasmid#222469PurposeExpresses hHus1 in insect cellsDepositorInserthHus1 (HUS1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His10TEV
Plasmid#159427Purposeempty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneUseTagsTEV-protease-cleavable His10-tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFnCpfAb-apr
Plasmid#214198PurposeExpresses FnCpf1 and RecAb recombination system for genome editing in Acinetobacter baumanniiDepositorInsertFnCpf1
UseCRISPRTagsExpressionMutationPromotertac promoterAvailable sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-pGC-A
Plasmid#186626PurposepFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)DepositorInsertNPR1 (NPR1 Human)
UseTagshemagglutinin (HA) signal peptide + His10-tag + T…ExpressionInsectMutationdeleted amino acids 1-32PromoterpolyhedringAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_SNF2L-opt_p48
Plasmid#102243PurposeExpression of human codon optimized human SNF2L and pRbAp48 from pFastBac dualDepositorUseTagsExpressionInsectMutationcodon optimized for expressionPromoterPolyhedrin and p10Available sinceNov. 1, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits