We narrowed to 542 results for: prime editing

1. pCMV-CMP-PE-V1

   Plasmid

   #202769

   Purpose

   Expresses CMP-PE-V1 in mammalian cells for prime editing

   Depositor

   Kyoungmi Kim

   Article

   Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

   Insert

   CMP-PE-V1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiGuide-BlastR

   Plasmid

   #196970

   Purpose

   Lentiviral acceptor vector for pegRNAs with blasticidin resistance

   Depositor

   Leopold Parts

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-CMV-PEmax-IRES-ZeoR

   Plasmid

   #207353

   Purpose

   Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   Prime editor max (PEmax) enzyme
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-CMV-PE2-IRES-ZeoR

   Plasmid

   #207352

   Purpose

   Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   Prime editor 2 (PE2) enzyme
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-PEmax-P2A-hMLH1dn

   Plasmid

   #174828

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-hMLH1dn
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript; deletion of residues 754-…

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-PEmax-P2A-GFP

   Plasmid

   #180020

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-EGFP
   

   Use

   Tags

   P2A-EGFP, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PEmax-P2A-BSD

   Plasmid

   #174821

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-BSD
   

   Use

   Tags

   P2A-BSD, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PE2-P2A-hMLH1dn

   Plasmid

   #174827

   Purpose

   Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PE2-P2A-hMLH1dn
   

   Use

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript; deletion of residues 754-…

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-PE2max-BSD

   Plasmid

   #191102

   Purpose

   Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   PEmax-P2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed R221K, N394K, H840A from SpCas9

   Promoter

   EF-1a

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE-Plus

    Plasmid

    #214079

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-NRCH-PE2max-BSD

    Plasmid

    #191103

    Purpose

    Lentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    NRCH-PEmax
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed R221K, N394K, H840A from SpCas9-NRCH

    Promoter

    EF-1a

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-PE2-BSD

    Plasmid

    #161514

    Purpose

    Expression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selection

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Sep 21. pii: 10.103

    Insert

    PE2-P2A-BSD
    

    Use

    Lentiviral

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α core promoter

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. rnLbCas12a-PE2

    Plasmid

    #213746

    Purpose

    For circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    rnLbCas12a-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R, H759A, R1138A in LbCas12a

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PE2-tagRFP-BleoR

    Plasmid

    #192508

    Purpose

    Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.

    Depositor

    Gerald Schwank

    Article

    Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    PE2-P2A-tagRFP-P2A-BleoR
    

    Use

    Tags

    BleoR, SV40 bpNLS, and tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. iPEmax-WT

    Plasmid

    #239385

    Purpose

    For inverse prime editor using Cas9-R221K-N394K in HEK294T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

    Insert

    Cas9-R221K-N393K, M-MLV-RT
    

    Use

    CRISPR

    Tags

    BPNLS and SV40 NLS, c-myc NLS

    Expression

    Mammalian

    Mutation

    R221K, N394K in Cas9

    Promoter

    CMV

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. iPEmax-D10A

    Plasmid

    #239386

    Purpose

    For inverse prime editor using nCas9-D10A-R221K-N394K in HEK295T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

    Insert

    nCas9-D10A-R221K-N394K, M-MLV-RT
    

    Use

    CRISPR

    Tags

    BPNLS and SV40 NLS, c-myc NLS

    Expression

    Mammalian

    Mutation

    D10A, R221K, N394K in Cas9

    Promoter

    CMV

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6:3-tevopreq1

    Plasmid

    #190909

    Purpose

    Expression of single epegRNA under control of Drosophila U6:3 promoter. Can be used to generate transgenic flies with vermillion+ selection.

    Depositor

    Norbert Perrimon

    Insert

    Empty Backbone
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6-tevopreq1-GG-acceptor

    Plasmid

    #174038

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-PE7-SB

    Plasmid

    #228869

    Purpose

    PE7 connected to MLH1-SB via 2A sequence

    Depositor

    Sangsu Bae

    Article

    Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

    Insert

    PE7-2A-MLH1-SB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6-tmpknot-GG-acceptor

    Plasmid

    #174039

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge