Addgene

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pClneoMyc'-EGL-44

Plasmid

#66855

Purpose

Express Myc-tagged C.elegans EGL-44 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

EGL-44 (CELE_F28B12.2 Nematode)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pClneoEGFPC-let60 K16N

Plasmid

#66861

Purpose

Express GFP-tagged dominat negative let60, in which Lysine 16 is mutated to asparagine, in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

Let-60 (let-60 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

Lysine 16 is mutated to asparagine

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pFM101

Plasmid

#40147

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

gfp::par-2 wild-type RNAi resistant (par-2 Nematode)

Use

Tags

GST and eGFP

Expression

Bacterial

Mutation

Base pairs 6-523 of par-2 were recoded to create …

Promoter

tac

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pFM102

Plasmid

#40148

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

gfp::par-2 RNAi resistant R183-5A (par-2 Nematode)

Use

Tags

GST and eGFP

Expression

Bacterial

Mutation

R183-5A; base pairs 6-523 of par-2 were recoded t…

Promoter

tac

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pHW394 (15xUAS::GFP::let-858 3'UTR)

Plasmid

#85584

Purpose

cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

15xUAS-delta pes-10-GFP

Use

Tags

Expression

Worm

Mutation

GFP(S65C) with three introns for expression in C.…

Promoter

15xUAS-delta pes-10-GFP

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}

Plasmid

#232880

Purpose

Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor.

Depositor

Paul DeCaen

Article

DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

Insert

ADP-ribosylation factor-like protein 13B (ARL13B Human)

Use

Lentiviral

Tags

GFP and RGECO1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)

Plasmid

#193048

Purpose

Heatshock expressed Cas9

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

hsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

hsp16.41p

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

Plasmid

#154824

Purpose

(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

Peft-3::wrmScarlet::tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

eef-1A.1 (eft-3)

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGM1190

Plasmid

#69994

Purpose

OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pJR019

Plasmid

#198834

Purpose

Inactivation of G protein subunits through Pertussis toxin (PTX)

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::PTX-SL2-GFP::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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