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Plasmid#108570PurposeExpression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factorsDepositorInsertdSpyCas9
UseCRISPR and LentiviralTagsAPEX2, Destabilization domains, Flag, and mCherry…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)
Plasmid#149722PurposeLentiviral expression of crRNA with GFP and a neomycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
ATGL*-V5-APEX2
Plasmid#170574PurposeA C-terminal APEX2 fusion to the human ATGL S47A proteinDepositorInsertATGL* (PNPLA2 Human)
UseLentiviralTagsV5-APEX2ExpressionMammalianMutationchanged serine 47 to alaninePromoterCMVAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Aqp4-BioID2-BioID2-HA
Plasmid#176742PurposeExpresses Aquaporin-4 fused with BioID2 at the C-terminus in astrocytesDepositorInsertAqp4-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-HA-BioID2-SAPAP3
Plasmid#176857PurposeExpresses SAPAP3 protein fused with BioID2 at the N-terminus in neuronsDepositorInsertHA-BioID2-SAPAP3
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPARC-bio-His
Plasmid#52078PurposeExpresses full-length Secreted protein acidic and rich in cysteine precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSPARC (SPARC Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Rac1-2G
Plasmid#66111PurposeSecond generation Rac1 FRET biosensor for lentivirus productionDepositorInsertSecond generation Rac1 FRET biosensor
UseLentiviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3*HA-Palmd
Plasmid#216833Purposeexpressing HA tag and the coding sequence of mouse PalmdDepositorInsertPalmd (mus musculus) (Palmd Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
PDIA4-bio-His
Plasmid#52071PurposeExpresses full-length Protein disulfide-isomerase A4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPDIA4 (PDIA4 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
TPP1-bio-His
Plasmid#52090PurposeExpresses full-length Tripeptidyl-peptidase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTPP1 (TPP1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-LiMETER-v1
Plasmid#113933PurposeExpress an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFPDepositorInsertLOV2 and Rit polybasic domain (PB)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Lck-13xLinker-BioID2-BioID2-HA
Plasmid#176741PurposeExpresses membrane bound biotin ligase, BioID2, in astrocytesDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-NES-L2
Plasmid#127351Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP
Plasmid#182825PurposeCre-dependent expression of H2B-APEX fusionDepositorInsertH2B.APEX-P2A-EGFP
UseAAVTagsEGFP reporter (P2A)ExpressionMutationPromoterEF1aAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
PDIA5-bio-His
Plasmid#52072PurposeExpresses full-length Protein disulfide-isomerase A5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPDIA5 (PDIA5 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
NgOE FUNgGW
Plasmid#92231PurposeLentiviral expression of human NgDepositorInsertNg (NRGN Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1-HA
Plasmid#137766PurposeExpression and localization of tagged CALCOCO1DepositorInsertCALCOCO1 (CALCOCO1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-CREB
Plasmid#82721PurposeExpresses human CREBDepositorInsertCREB (CREB1 Human)
UseTags3XFLAG and GSTExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA
Plasmid#176854PurposeExpresses membrane bound biotin ligase, BioID2, in neuronsDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
ULBP3-bio-His
Plasmid#51859PurposeExpresses full-length NKG2D ligand 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertULBP3 (ULBP3 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only