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Plasmid#100765PurposeLentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imagingDepositorInsertLenti-Mito-Car-GECO
UseLentiviralTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationR-GECO1: E163V/I166V/V174T/M176I/F222I/A302PPromoterCMVAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGS_128
Plasmid#160651PurposeExpresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoterDepositorInsertApolipoprotein E e3 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione3 allelePromoterpZ estradiol inducible promoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR-BLINK2
Plasmid#117075PurposeBLINK2 gene in pDONR vector for Gateway cloning systemDepositorInsertBLINK2
UseGateway cloning systemTagsExpressionMutationglutamine 112 changed into aspartate (original LO…PromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_101
Plasmid#160541PurposeExpresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoterDepositorInsertApolipoprotein E e4 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione4 allelePromoterGAL1Available sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGS_107
Plasmid#160650PurposeExpresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoterDepositorInsertApolipoprotein E e4 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione4 allelePromoterGAL1Available sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGS_129
Plasmid#160652PurposeExpresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoterDepositorInsertApolipoprotein E e4 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione4 allelePromoterpZ estradiol inducible promoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-INPP5E-CAAX
Plasmid#202746Purposeexpress plasma membrane targeted fluorescent protein-tagged enzymeDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 blast OFF 3xflag IREB2
Plasmid#169889PurposeExpress 3x flag IREB2, suppressible by doxycycline additionDepositorInsertIREB2 (IREB2 Human)
UseLentiviral; Doxycycline mediated suppressionTags3x FlagExpressionMammalianMutationSilent mutations in sgIREB2_1 and sgIREB2_2 targe…PromoterTREAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer10b-EGFP-KRAS-WT
Plasmid#164935PurposeExpresses EGFP cDNA and KRAS wild type cDNA in mammalian cellsDepositorInsertsUseLentiviralTagsExpressionMutationPromoterUbc Promoter and Ubc promoterAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-PHD2-181-426
Plasmid#223551PurposeHis-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purificationDepositorInsertPHD2 (EGLN1 Human)
UseTags6x HisExpressionBacterialMutationDeleted amino acids 1-180.PromotertrcAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 ISCU D71A
Plasmid#169880PurposeExpresses ISCU iron-sulfur cluster trapping mutant (dominant negative)DepositorInsertISCU (ISCU Human, Synthetic)
UseLentiviralTagsExpressionMammalianMutationCodon Optimized, D71A iron-sulfur cluster trappin…PromoterTREAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_Hu_ECT2(WT)
Plasmid#136330PurposeLentiviral expression of human ECT2 WTDepositorInsertECT2 (ECT2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEFL GAL4dbd-KLF4 1-329
Plasmid#140146PurposeGAL4-DNA binding domain fusion protein with the 329 N-terminal amino acids of human KLF4DepositorInsertKLF4 Kruppel factor (KLF4 Human)
UseTagsGAL4dbdExpressionMammalianMutationDeleted amino acids 330 to 479PromoterEF1aAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP GFP-MOSPD2 W201E
Plasmid#186471PurposeExpression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to EGFP in mammalian cellsDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationW201E (no more binding to lipid droplets)PromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEFL GAL4dbd-KLF4 1-156
Plasmid#140147PurposeGAL4-DNA binding domain fusion protein with the 156 N-terminal amino acids of human KLF4 (activation domain)DepositorInsertKLF4 aa 1-156 (KLF4 Human)
UseTagsGAL4dbdExpressionMammalianMutationDeleted amino acids 157 to 479PromoterEF1aAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUMO mouse METAP1 1-79
Plasmid#197397PurposeExpresses SUMO-tagged mouse METAP1, residues 1-79DepositorInsertMETAP1 1-79 (Metap1 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUMO mouse METAP1 1-59
Plasmid#197396PurposeExpresses SUMO-tagged mouse METAP1, residues 1-59DepositorInsertMETAP1 1-59 (Metap1 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_Hu_ECT2(T327D)
Plasmid#136332PurposeLentiviral expression of human ECT2_T327DDepositorInsertECT2 (ECT2 Human)
UseLentiviralTagsExpressionMammalianMutationT327DPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TKN-bp1
Plasmid#133428PurposeHuman BP1 fragment of SREBF1 in vector for bacterial expression of GST fusion protein.DepositorInsertSREBF1 (SREBF1 Human)
UseTagsExpressionBacterialMutationSREBP1a amino acids 1-50 fused to GSTPromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-FBXL5-SKP1
Plasmid#226620PurposeCo-expression of FBXL5-SKP1 complex in E.coliDepositorUseTagsGB1ExpressionBacterialMutationAmino acids 1-198 was deleted, and 420-596 was re…PromoterT7Available sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only