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Showing: 981 - 1000 of 13534 results

  1. pclbw-PAGFPomp25

    Plasmid
    #58427
    Purpose
    Mammalian expression of photoactivatable GFP targeted to the mitochondrial outer membrane
    Depositor
    David Chan
    Article
    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi
    Insert
    paGFP-omp25 (Synj2bp Mouse)
    Use
    Retroviral
    Tags
    PAGFP
    Expression
    Mammalian
    Mutation
    contains aa's 109-145 of mouse omp25
    Promoter
    cmv-bactin
    Available since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. HeFm2SpCas9

    Plasmid
    #92111
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    “Highly enhanced Fidelity” mut2 SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92111-plasmid-map-sequence-id-197616

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  4. Keasling-2337

    Plasmid
    #100168
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    lacIq-Ptrc-ADS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100168-plasmid-map-sequence-id-193496

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  9. pSR04

    Plasmid
    #69151
    Purpose
    read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV
    Depositor
    Sander van den Heuvel
    Article
    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10
    Inserts
    mCherry
    TagBFP
    eGFP
    Use
    Cre/Lox; Mossci
    Tags
    Expression
    Worm
    Mutation
    codon-optimzed index 1.0
    Promoter
    eef-1A.1 (eft-3) and rps-27
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69151-plasmid-map-sequence-id-125046

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  13. pMV306DIG13+FFlucRT

    Plasmid
    #49998
    Purpose
    Integrase removed to increase stability of the plasmid in mycobacteria
    Depositor
    Brian Robertson
    Article
    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2
    Insert
    G13 promoter + Firefly luciferase Red Thermostable
    Use
    Mycobacteria integrating vector
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for Mycobacterium tuberculosis wi…
    Promoter
    G13
    Available since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    49998-plasmid-map-sequence-id-74812

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  17. pCAGGS SARS-CoV-2 XBB Spike

    Plasmid
    #195287
    Purpose
    Expresses codon-optimized full length SARS-CoV-2 XBB Spike
    Depositor
    Marceline Côté
    Insert
    SARS-CoV-2 XBB Spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains the following mutations: T19I, LPPA24-27…
    Promoter
    chicken β-actin promoter, CMV enhancer
    Available since
    Feb. 8, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195287-plasmid-map-sequence-id-384139

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  19. pCRISPR-aBEST

    Plasmid
    #131464
    Purpose
    Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes
    Depositor
    Tilmann Weber
    Article
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Inserts
    Streptomyces codon optimized spCas9n (D10A)
    streptomyces codon optimized tadA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    tipA
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    131464-plasmid-map-sequence-id-261686

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  20. RGB-S reporter

    Plasmid
    #207841
    Purpose
    A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli
    Depositor
    Christof Niemeyer
    Article
    Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(
    Inserts
    RpoH sensing construct
    SOS sensing construct
    RpoS sensing construct
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PosmY, PsulA, and grpE
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207841-plasmid-map-sequence-id-426759

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Showing: 981 - 1000 of 13534 results