We narrowed to 14,422 results for: nts

1. pcDNA3.1-mCherry-CyrCR1.miR16

   Plasmid

   #128747

   Purpose

   Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Cyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Fireworks GFP[PTC+] (AVA2600)

   Plasmid

   #85446

   Purpose

   Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

   Depositor

   Joan Steitz

   Article

   Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

   Insert

   tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
   

   Use

   Minimal backbone fragment for low-copy bacterial …

   Expression

   Mammalian

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS NL4-3 envFS eGFP Δrev

   Plasmid

   #101348

   Purpose

   All mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Mutation

   delta rev

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21c(+)-mtPheRS

   Plasmid

   #31080

   Depositor

   Linda Spremulli

   Article

   Bullard et al J Mol Biol. 1999 May 14. 288(4):567-77.

   Insert

   Mature form human mitochondrial phenylalanine-tRNA synthetase (FARS2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Includes coding sequence for the mature form. Nt …

   Promoter

   T7

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCDF-NtXNtTcR1AtR2NtB2

   Plasmid

   #160889

   Purpose

   Expression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   PT7-Nt-rbcS-Tc
   

   Expression

   Bacterial

   Promoter

   rbcS-Tc: T7, chaperones: T12

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDF-NtXNtT2R1AtR2NtB2

   Plasmid

   #160886

   Purpose

   Expression of N. tabacum Rubisco small subunit (rbcS-T2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   PT7-Nt-rbcS-T2
   

   Expression

   Bacterial

   Promoter

   rbcS-T2: T7, chaperones: T9

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. SGL40C-H1.EFS.RFP657

   Plasmid

   #69148

   Purpose

   Advanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Inserts

   sgRNA
   EFS
   tagRFP657
   

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Tag 3B/WT myc-M87

   Plasmid

   #87722

   Purpose

   Expresses WT myc-M87 spastin isoform in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

   Insert

   SPAST (SPAST Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   M87 isoform (deletion of nt 1-490 - 5'UTR an…

   Promoter

   CMV

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. HOXB13 (human) MSCV

   Plasmid

   #8544

   Depositor

   Corey Largman

   Article

   Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

   Insert

   HOXB13 (HOXB13 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   contains 141 NT of 5' UTR prior to ATG start

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

10. SGL40C.EFS.RFP657

    Plasmid

    #69147

    Purpose

    Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with RFP657 expression (EFS Promoter)

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    sgRNA
    EFS
    tagRFP657
    

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. TR- ATG-HP-GFP

    Plasmid

    #80592

    Purpose

    Expresses GFP with an Csy4 target hairpin inserted immediately following the start codon for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    GFP
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Inserted 30 nt hairpin which is targeted by Csy4 …

    Promoter

    CBA

    Available Since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. TR-5'UTR-HP-GFP

    Plasmid

    #80594

    Purpose

    Expresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    GFP
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Inserted 30 nt hairpin which is targeted by Csy4 …

    Promoter

    CBA

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDF-NtXNtT5R1AtR2NtB2

    Plasmid

    #160888

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-T5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    PT7-Nt-rbcS-T5
    

    Expression

    Bacterial

    Promoter

    rbcS-T5: T7, chaperones: T11

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDF-NtXNtT1R1AtR2NtB2

    Plasmid

    #160885

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-T1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    PT7-Nt-rbcS-T1
    

    Expression

    Bacterial

    Promoter

    rbcS-T1: T7, chaperones: T8

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. HOXC6-pCT

    Plasmid

    #21000

    Depositor

    Corey Largman

    Article

    Lawrence et al Cell Growth Differ. 1993 Aug . 4(8):665-

    Insert

    HOXC6 (HOXC6 Human)
    

    Expression

    Mammalian

    Mutation

    Long 5' UTR, approx 480 nt

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-mCherry-CyrCR1.miR17

    Plasmid

    #128748

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-17 site inserted in mCherry 3'UTR.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with miR-17 site replacing miR-7 site (OIP5-AS1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRII-Topo Crym in situ probe

    Plasmid

    #45601

    Depositor

    Jeffrey Macklis

    Article

    Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

    Insert

    Crym in situ probe (Crym Mouse)
    

    Use

    In situ

    Mutation

    fragment contains nt 756-1191 of NM_016669

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

18. HOXD13 (chicken) HIS-tag pET

    Plasmid

    #8555

    Depositor

    Corey Largman

    Article

    Shen et al J Biol Chem 1997 Mar 28;272(13):8198-206

    Insert

    HOXD13 (HOXD13 )
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    contains 10 nt 5' to ATG start; makes prote…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pCRII-Topo Inhba

    Plasmid

    #45868

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    Inhba in situ probe (Inhba Mouse)
    

    Use

    In situ

    Mutation

    nt 428-933 from Inhba mRNA (NM_008380.1)

    Available Since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

20. TL1 alphaDdCBE 3_NC (right, DddA11-C)

    Plasmid

    #222005

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_NC right)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: T1413I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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