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p7XNH3

Plasmid

#47064

Purpose

FX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pET28CLY5

Plasmid

#21764

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 5 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pMOTag-glmS-4H

Plasmid

#106378

Purpose

Can be used as a PCR template to amplify the glmS-HA fragment for gene specific tagging and regulation

Depositor

Roberto Docampo

Article

Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:

Insert

GlmS-3xHA

Available Since

March 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pME eos

Plasmid

#73792

Purpose

Photoconvertible eos in a middle entry clone

Depositor

David Raible

Article

Prendergast et al Development. 2012 Mar;139(6):1141-52. do

Insert

EOS

Use

Middle entry clone

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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p7XC3H

Plasmid

#47065

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pPsrA-R1

Plasmid

#74688

Purpose

PsrA-R1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSR1-PsrA-PPsrA-YFP

Expression

Bacterial

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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pPhlF-P2

Plasmid

#74686

Purpose

PhlF-P2 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSP2-PhlF-PPhlF-YFP

Expression

Bacterial

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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pBetI-E1

Plasmid

#74677

Purpose

BetI-E1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSE1-BetI-PBetI-YFP

Expression

Bacterial

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-SaNAS

Plasmid

#110803

Purpose

Expresses SaNAS a nicotianamine synthase from Staphylcoccus aureus

Depositor

Audrey Lamb

Article

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

SAV2469 (SAV2469 )

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-PaNAS

Plasmid

#110801

Purpose

Expresses PaNAS nicotianamine synthase from pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

Pa4836

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-SaODH

Plasmid

#110804

Purpose

Expresses SaODH and opine dehydrogenase from Staphylococcus aureus

Depositor

Audrey Lamb

Article

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

SAV2468 (SAV2468 )

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLew100_myc_BirA*

Plasmid

#41716

Depositor

Graham Warren

Article

Morriswood et al Eukaryot Cell. 2012 Dec 21.

Type

Empty backbone

Use

T. brucei expression

Promoter

PARP

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pET28CLY6

Plasmid

#21765

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 6 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pBXC3H

Plasmid

#47068

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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p7XC3GH

Plasmid

#47066

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pEBB-TB

Plasmid

#36099

Depositor

Ezra Burstein

Article

Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

Type

Empty backbone

Tags

BT-tag

Expression

Mammalian

Promoter

EF1a

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only

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pET28CLY7

Plasmid

#21766

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 7 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pQE-WT-KIF-R5-IRH

Plasmid

#63629

Purpose

K1F-R5-IRH RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

KIF-R5-IRH RNAP

Mutation

L749I, Q754S, R756N, I761V, H772R, and Q786H

Promoter

T5/Lac

Available Since

June 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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NK612

Plasmid

#109092

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

Lifeact-mCherry

Use

Choanoflagellate expression

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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RGG-NanoLuc-RGG

Plasmid

#176642

Purpose

NanoLuc enzyme fused to LAF-1 RGG domain

Depositor

Daniel Hammer

Article

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

RGG-NanoLuc-RGG

Expression

Bacterial

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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