We narrowed to 11,712 results for: NSI

1. 5HT6-CFP-Venus(H148G)

   Plasmid

   #47501

   Purpose

   Fluorescent reporter for pH inside primary cilia

   Depositor

   Takanari Inoue

   Article

   Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

   Insert

   Venus(H148G)
   

   Tags

   5HT6 and CFP

   Expression

   Mammalian

   Mutation

   H148G mutation

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pGWT35S-Cerulean-SKL

   Plasmid

   #194048

   Purpose

   Transient expression of Cerulean-SKL in plant cell (Peroxisome)

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

   Insert

   Cerulean
   

   Tags

   Ser-Lsy-Leu

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(−) mCER-Prx2

   Plasmid

   #158224

   Purpose

   Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for transient transfection in mammalian cells)

   Depositor

   Tobias Dick

   Article

   Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

   Insert

   mCER-Prx2
   

   Expression

   Mammalian

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632)

   Plasmid

   #70742

   Purpose

   1 color weak reporter used in study

   Depositor

   Richard Murray

   Article

   Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

   Insert

   sfGFP-ssrA
   

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPRIME-TREX-GFP-recipient

   Plasmid

   #11661

   Purpose

   3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with Tet-responsive promoter (TREX) controlling GFP expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUA66 PcspA GFP Linker

   Plasmid

   #105604

   Purpose

   Expresses GFP under temperature sensitive promoter PcspA

   Depositor

   Pamela Silver

   Article

   Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PcspA

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. p-dCas9-DNMT3a-Hygro

   Plasmid

   #104403

   Purpose

   transient expression of dCas9-DNMT3a fusion protein

   Depositor

   Stephan Beck

   Insert

   DNMT3a (DNMT3A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMUT2-pCaf-mCherry

   Plasmid

   #192860

   Purpose

   pMUT2 based vector for sensing caffeine in E. coli Nissle 1917

   Depositor

   Morten Sommer

   Article

   Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

   Insert

   Nanobody-CadC fusion protein and cognate promoter inducible by caffeine
   

   Expression

   Bacterial

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-HA ZNHIT3

   Plasmid

   #110953

   Purpose

   Transient expression of HA ZNHIT3

   Depositor

   David Sabatini

   Article

   Wyant et al Science. 2018 May 18;360(6390):751-758.

   Insert

   ZNHIT3 (ZNHIT3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJC225

    Plasmid

    #176883

    Purpose

    MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Cloning

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIS.MTRIA-AGT

    Plasmid

    #184598

    Purpose

    For expression of a fluorescent sensor for angiotensin II in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-AGT
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMZ2007

    Plasmid

    #204399

    Purpose

    A 3OC6-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

    Inserts

    quorum sensing regulator LuxR
    GFP
    

    Use

    Synthetic Biology

    Promoter

    PftsH and Synthetic promoter PLux0002

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pL0M-S-mClover3-EC18157

    Plasmid

    #137076

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mClover3 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBCC155

    Plasmid

    #74995

    Purpose

    CFP-based BiFC vector for transient expression of CC (155-238) in plants

    Depositor

    Yutaka Kodama, Masamitsu Wada

    Article

    Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

    Type

    Empty backbone

    Use

    Reporter plasmid

    Tags

    CFP (155–239)/CC155

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBCN155

    Plasmid

    #74994

    Purpose

    CFP-based BiFC vector for transient expression of CN (1-155) in plants

    Depositor

    Yutaka Kodama, Masamitsu Wada

    Article

    Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

    Type

    Empty backbone

    Use

    Reporter plasmid

    Tags

    CFP (1–155)/CN155

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMTP112 (pMS26_miniTn6900(KanR))

    Plasmid

    #164260

    Purpose

    Delivery vector to insert the miniTn6900(KanR) element into the E. coli chromosome at the neutral Tn7 attachment site inside a miniTn7 element.

    Depositor

    Joseph Peters

    Article

    Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

    Insert

    miniTn6900 element
    

    Use

    Bacterial delivery vector for tn7 att site

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBtet-Bla/PAG1-TurboID-GAI

    Plasmid

    #198217

    Purpose

    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling

    Depositor

    Eric Davis

    Insert

    PAG1 (PAG1 Human)
    

    Use

    Transposon

    Tags

    3’ part of TurboID, starting with G257 and amino …

    Expression

    Mammalian

    Mutation

    None

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3918-37

    Plasmid

    #176495

    Purpose

    pTRE-EGFP-T2A-mCherry-polyA

    Depositor

    Albert La Spada

    Article

    Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

    Insert

    Tetracyline Responsive Element driving EGFP-T2A-mCherry
    

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAM5089

    Plasmid

    #85125

    Purpose

    KaiB expressed from native promoter from NSII-Nt. Complements rhythms of kaiB null strain.

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    PkaiB-kaiB
    

    Expression

    Bacterial

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMUT2-pAto-mCherry

    Plasmid

    #192858

    Purpose

    pMUT2 based vector for sensing acetoacetate in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    Acetoacetate inducible promoter
    

    Expression

    Bacterial

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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