We narrowed to 19,308 results for: Tat

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pcDNA-hDDB2-K244E-V5

Plasmid

#167471

Purpose

ectopic expression of hDDB2 in mammalian cells

Depositor

Steven Artandi

Article

Chen et al DNA Repair (Amst). 2021 Jan;97:103022. d

Insert

DDB2 (DDB2 Human)

Tags

6xHis and V5

Expression

Mammalian

Mutation

K244E

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST15-Nedd4.2F823A

Plasmid

#165104

Purpose

bacterial expression of a GST fusion of Human Nedd4.2d F823A

Depositor

Arthur Haas

Article

Todaro et al J Biol Chem. 2018 Nov 23;293(47):18192-1

Insert

Nedd4.2d F823A (NEDD4L Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

F823A; mutation for destabilization of trimer fo…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI757 P(PGK1)-citrine P(HIS4)-mCherry

Plasmid

#164917

Purpose

Divergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizer

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(PGK1)-citrine and P(HIS4)-mCherry

Expression

Yeast

Promoter

PGK1 (yeast) and HIS4 (yeast)

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Apob-2

Plasmid

#162549

Purpose

sgRNA targeting Apob

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Apob (Apob Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 K395A/K396A

Plasmid

#162656

Purpose

Expresses ORF68 K395A/K396A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acids K395 and K396 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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BE3-hA3A-Y130F

Plasmid

#132945

Purpose

Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

Depositor

Hui Yang

Article

Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

Insert

BE3-hA3A(Y130F)

Use

CRISPR; Base editor

Expression

Mammalian

Mutation

BE3-hA3A(Y130F)

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pEF Doc2beta 6X mutant GFP

Plasmid

#128823

Purpose

To visualize Doc2beta 6X mutant in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

D163A, D218A, D220N, D303A, D357A, D359A

Promoter

EF-1alpha

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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mOrange-P2A-hKvb1

Plasmid

#154084

Purpose

Independent expression of mOrange and human Kvbeta 1 under human synapsin 1 promoter

Depositor

Michael Hoppa

Article

Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

Insert

mOrange-P2A-human Kvb1 (KCNAB1 Human, Synthetic)

Use

AAV

Expression

Mammalian

Promoter

human synapsin 1

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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p6H-SUMO3-ORF24-NTD-Strep

Plasmid

#138467

Purpose

Expresses SUMO3-tagged ORF24-NTD-Strep in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

SUMO3 and Strep-Tag II

Expression

Bacterial

Mutation

Amino acids 2-201

Promoter

T7

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-UAP56 MUT

Plasmid

#110124

Purpose

Expresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

UAP56 (DDX39B Human)

Tags

Flag

Expression

Insect

Mutation

4 mutations (KSLN motif changed to RSFS)

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR S286/287/291A

Plasmid

#159160

Purpose

Expresses DEPTOR S286/287/291A mutant in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Mutation

changed serine 286,287 and 291 to alanine

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR S286/287/291D

Plasmid

#159161

Purpose

Expresses DEPTOR S286/287/291D mutant in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Mutation

changed serine 286,287 and 291 to aspartic acid

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-C-Flag-GK5_v2

Plasmid

#134282

Purpose

Expresses Flag-tagged GK5 (isoform 2) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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TRRAP sgRNA2

Plasmid

#138183

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRRAP

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TRRAP (TRRAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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B-eSpCas9

Plasmid

#126761

Purpose

Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

B-eSpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

K848A; K1003A; R1060A; amino acids 1005-1013 repl…

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9

Plasmid

#126765

Purpose

Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

B-evoSpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

M495V; Y515N; K526E; R661Q; amino acids 1005-1013…

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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ETV7_A86D_pet28a

Plasmid

#131657

Purpose

Bacterial expression of ETS gene with A86D mutation for solubility

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV7 A86D (ETV7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Alanine 86 to Aspartic Acid

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pmKO-AF8

Plasmid

#118224

Purpose

Kusabira- Orange-CENP-A expression plasmid

Depositor

Kenji Sugimoto

Article

Sugimoto et al Mutat Res. 2008 Nov 17;657(1):56-62. doi

Insert

CENP-A (CENPA Human)

Tags

Kusabira orange

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP III (AA 1-84)

Plasmid

#119319

Purpose

lentiviral vector for expression of HA-Tagged E6-AP III AA 1-84 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP III (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 1-84

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 1-500)

Plasmid

#119312

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 1-500 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 1-500

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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