We narrowed to 18,247 results for: multi
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Plasmid#171083PurposeCo-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permissionDepositorTagsHisExpressionBacterialPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only
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MRGPRX4-DuET
Plasmid#213347PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR156-DuET
Plasmid#213267PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
AGTR2-DuET
Plasmid#213184PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ULK1 sgRNA
Plasmid#207559PurposepX330 expressing Cas9 and a sgRNA targeting the ULK1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCCAGCCAGGCCAGAAAGGTC
ExpressionMammalianPromoterCMV and U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MRGPRG-DuET
Plasmid#213343PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR63-DuET
Plasmid#213289PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATG5 sgRNA
Plasmid#207555PurposepX330 expressing Cas9 and a sgRNA targeting the ATG5 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAACTTGTTTCACGCTATATC
ExpressionMammalianPromoterCMV and U6Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATG9A sgRNA
Plasmid#207557PurposepX330 expressing Cas9 and a sgRNA targeting the ATG9A locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCACTGAATACCAGCGCCTAG
ExpressionMammalianPromoterCMV and U6Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TGA
Plasmid#208189Purposenonsense mistranslation, TGA stopDepositorInsertsGFPmut2
mScarlet-I
UseReporterExpressionBacterialMutationInsertion 6TGA*(stop) 7AGT(Ser)PromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TAA
Plasmid#208187Purposenonsense mistranslation, TAA stopDepositorInsertsGFPmut2
mScarlet-I
UseReporterExpressionBacterialMutationInsertion 6TAA*(stop) 7AGT(Ser)PromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NLS-FRB-VP16
Plasmid#210515Purposeexpresses NLS-FRB-VP16 component in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-FRB-VP16
ExpressionMammalianPromoterCMVAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV298
Plasmid#204957PurposeAMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection markerDepositorInsertsMad7
pyrG
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and native pro…Available sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lepr peak reporter-eGFP
Plasmid#196584PurposeCis-regulatory region of mouse Lepr gene driven a eGFP reporter with hGFP driven rtTA3DepositorInserteGFP
UseLentiviral and RNAiExpressionMammalianPromoterSV40Available sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0014
Plasmid#205583PurposeHYG(1-89)-NpuDnaE(N)_GFPuvDepositorInsertNpuDnaE(N)
ExpressionPlantPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
RS35075_pMQ30_KO_Kan
Plasmid#185390PurposeNon-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11DepositorInsertsRR42_RS35075 upstream flanking homology region
RR42_RS35075 downstream flanking homology region
nptII kanamycin resistance marker
ExpressionBacterialAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RRVQ
Plasmid#200188PurposeGateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editingDepositorInsertLbCas12a-RRVQ
UseCRISPR; Gateway compatible lbcas12a-rrv entry clo…TagsNLSExpressionPlantMutationG146R, D156R, R182V, E795QAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG 3.2
Plasmid#193803PurposeProtein production of ChemoG3.2 in bacteriaDepositorInsertChemoG 3.2
Tags10xHisExpressionBacterialMutationEGFP:A206K, T225R; HT7: L271EPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoR-ATP
Plasmid#193820PurposeCMV overexpression of ChemR-ATP in mammalian cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertChemoR-ATP
ExpressionMammalianPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMN-294
Plasmid#195453PurposeLentiviral Integratable mCherry Reporter for synZiFTR ZF1DepositorInsert8X ZF1 BS - ybTATA - mCherry
UseLentiviralExpressionMammalianAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only