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pT7sgRNA

Plasmid

#111820

Purpose

Expresses sgRNA in T. brucei cells

Depositor

David Horn

Article

Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

Insert

sgRNA

Use

CRISPR

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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PKC alpha dNPS

Plasmid

#21233

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC alpha (PRKCA Human)

Tags

HA

Expression

Mammalian

Mutation

delta 1-29

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
pICH51288

Plasmid

#50269

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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Degron-KI SF3B1 donor vector

Plasmid

#65622

Purpose

Donor vector to generate Degron-KI DD-SF3B1

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

SF3B1 Degron-KI left and right HR arms

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pHACK(Gal4)-DONR(T2A-LexAGAD)

Plasmid

#194769

Purpose

To insert T2A-LexAGAD into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-LexAGAD transgene.

Depositor

Chun Han

Article

Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

Insert

T2A-LexAGAD

Use

CRISPR

Expression

Insect

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pH3FS

Plasmid

#85780

Purpose

N-ICE plasmid pH3FS

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pUC_sgRNA

Plasmid

#68710

Purpose

Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

Depositor

Roberto Docampo

Article

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Available Since

Sept. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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EC67897

Plasmid

#209491

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a-tRNA-DR-DR

Use

CRISPR

Promoter

HvU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC67899

Plasmid

#209493

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a-tRNA-DR-DR

Use

CRISPR

Promoter

HvU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70130

Plasmid

#209501

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a-tRNA-DR-DR

Use

CRISPR

Promoter

TaU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-146a

Plasmid

#64234

Purpose

Expresses miR-146a in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR146a (MIR146A Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ6332 - ACTB CRISPRa CLIP Donor

Plasmid

#210015

Purpose

CLIP donor for integrating CRISPRa (dCas12-miniVPR)-T2A-GFP-P2A to the ACTB locus

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

dLbCpf1-miniVPR-T2A-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Golden-Gate-Esp3I-Destination

Plasmid

#51278

Purpose

CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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NCD-GFP

Plasmid

#24507

Depositor

Ron Vale

Article

Case et al Cell. 1997 Sep 5. 90(5):959-66.

Insert

NCD (ncd Fly)

Tags

GFP and His

Expression

Bacterial

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-miR17

Plasmid

#21113

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

miR-17 (MIR17 Human)

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
pTH650-RLuc/staFLuc

Plasmid

#29696

Depositor

Tobias von der Haar

Article

Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

Insert

Firefly Luciferase

Expression

Yeast

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG-b3STOPb3-ZsGreen

Plasmid

#51266

Purpose

B3-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by B3-recognition sites followed by ZsGreen CDS

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

b3STOPb3-ZsGreen

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pME-Ruby2-NS

Plasmid

#166569

Purpose

Multisite Gateway middle entry clone encoding mRuby2. Parton lab clone DJA

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Ruby2

Use

Gateway Cloning

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pT3TS-BASU-EGFP

Plasmid

#163845

Purpose

In vitro transcription of BASU-EGFP. Parton lab clone JRR

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

BASU

Use

In vitro transcription of rna

Tags

EGFP

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-rSyntaxin-3a

Plasmid

#195206

Purpose

Mammalian expression vector for GFP tagged Syntaxin-3a.

Depositor

David Bryant, Thomas Weimbs

Article

Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

Insert

STX3A (Stx3 Rat)

Expression

Mammalian

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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