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Showing: 10021 - 10040 of 18162 results

  2. B-Sniper SpCas9

    Plasmid
    #207361
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-Sniper SpCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207361-plasmid-map-sequence-id-410129

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  3. pLenti-C-Myc-DDK-IRES-Puro HNRNPR

    Plasmid
    #200730
    Purpose
    Lentiviral expression of Flag-Tagged human HNRNPR
    Depositor
    Claudio Alarcón
    Insert
    HNRNPR (HNRNPR Human)
    Use
    Lentiviral
    Tags
    Myc-DDK from backbone plasmid
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    200730-plasmid-map-sequence-id-409225

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  4. p667-UBC-ALPK2-V5-miniTurbo_IDG-K

    Plasmid
    #198009
    Purpose
    Gateway destination clone of ALPK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    ALPK2-V5-miniTurbo (ALPK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    198009-plasmid-map-sequence-id-390040

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  7. Ef1a-DIO-Aion-citrine

    Plasmid
    #192571
    Purpose
    AAV-vector for optogenetic long-term silencing of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    Aion
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…
    Promoter
    Ef1-alpha
    Available Since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    192571-plasmid-map-sequence-id-382820

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  8. pHAGE-CMV-Flag-RIOK3-STOP_IDG-K

    Plasmid
    #170576
    Purpose
    Gateway destination clone of RIOK3 (human) tagged with N-terminal Flag for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    Flag-RIOK3 (RIOK3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    170576-plasmid-map-sequence-id-381720

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  9. mCherry-7aa-cb5-pEGFP-C1

    Plasmid
    #177407
    Purpose
    To express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5r4 Rat)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177407-plasmid-map-sequence-id-348154

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  10. p667-UBC-ADCK5-V5-miniTurbo_IDG-K

    Plasmid
    #189909
    Purpose
    Gateway destination clone of ADCK5 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    ADCK5 (ADCK5 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    189909-plasmid-map-sequence-id-379308

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  11. p663-UBC-miniTurbo-V5-NIM1K_IDG-K

    Plasmid
    #189892
    Purpose
    Gateway destination clone of NIM1K (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    NIM1K (NIM1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    189892-plasmid-map-sequence-id-374344

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  12. p663-UBC-miniTurbo-V5-STK3_IDG-K

    Plasmid
    #189879
    Purpose
    Gateway destination clone of STK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    STK3 (STK3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    189879-plasmid-map-sequence-id-374329

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  13. p667-UBC-SBK2-V5-miniTurbo_IDG-K

    Plasmid
    #189899
    Purpose
    Gateway destination clone of SBK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    SBK2 (SBK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    189899-plasmid-map-sequence-id-374024

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  14. p667-UBC-PKMYT1-V5-TurboID_IDG-K

    Plasmid
    #135247
    Purpose
    Gateway destination clone of PKMYT1 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    PKMYT1-V5-TurboID (PKMYT1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    135247-plasmid-map-sequence-id-371121

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  15. pCSThaP6ERT(#184)

    Plasmid
    #184103
    Purpose
    cyclofen-inducible mouse Pax6 activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    HA-MmPax6-ERT2
    Use
    Synthetic Biology
    Tags
    HA
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6+T7
    Available Since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184103-plasmid-map-sequence-id-365942

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  16. pT2iC6Dronpa2nlsER4(#636)

    Plasmid
    #184067
    Purpose
    cyclofen-inducible Dronpa2 nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    Dronpa2-2nls-ERT2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184067-plasmid-map-sequence-id-363960

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  18. isoform PKC Delta (JCE594)

    Plasmid
    #182025
    Purpose
    yeast expression of mouse PKC Delta with yeast PKC1 promoter and ADH1 terminator
    Depositor
    Juan Carlos Igual
    Insert
    PKC Delta (Prkcd Mouse)
    Expression
    Mammalian
    Promoter
    yeast PKC1 promoter (-600 to -1)
    Available Since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    182025-plasmid-map-sequence-id-361958

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  19. pAc5.1B-lambdaN-HA-DmBicaudalC_P

    Plasmid
    #147163
    Purpose
    Insect Expression of DmBicaudalC
    Depositor
    Elisa Izaurralde
    Insert
    DmBicaudalC (BicC Fly)
    Expression
    Insect
    Mutation
    12 silent and three non silent mutattions I331T, …
    Available Since
    March 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    147163-plasmid-map-sequence-id-355687

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  20. CD44I-c013

    Plasmid
    #175450
    Purpose
    Protein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Tags
    His6
    Expression
    Mammalian
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175450-plasmid-map-sequence-id-353226

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Showing: 10021 - 10040 of 18162 results