We narrowed to 46,018 results for: cha

Showing: 5021 - 5030 of 46018 results

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hCas9-VPR

Plasmid

#68497

Purpose

nuclease competent SP-Cas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

SP-Cas9-VPR

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

Plasmid

#35152

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5, pTrc

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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ZJOM153

Plasmid

#133642

Purpose

Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

Vph1-2GFP (VPH1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pOBD2

Plasmid

#196826

Purpose

Yeast two-hybrid ; Gal4 DNA binding domain

Depositor

Stan Fields

Article

Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

Type

Empty backbone

Tags

Gal4 DNA binding domain

Expression

Yeast

Promoter

ADH

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET-NtC60αC20NtLC60β

Plasmid

#160877

Purpose

Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, cpn20

Expression

Bacterial

Promoter

rbcL: T7, chaperones: T7

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMP11

Plasmid

#215559

Purpose

pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Inserts

Cas9
gRNA targeting pBR322 origin of replication

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23105 and tet

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-HphMX6

Plasmid

#240973

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HphMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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tubP-FRT>GAL80-6-FRT>

Plasmid

#63173

Purpose

ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"Flp-Out" GAL80

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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TPC1-G-GECO1.2

Plasmid

#207143

Purpose

Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

TPCN1 (TPCN1 Human)

Tags

G-GECO1.2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::CreERT2-bGHpA

Plasmid

#62444

Purpose

MXS_chaining vector with PGK::CreERT2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tamoxifen inducible Cre recombinase with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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