We narrowed to 14,477 results for: NTS

1. pcDNA3-N-Flag-NLRP3 711-1034

   Plasmid

   #75141

   Purpose

   Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   fragment encoding amino acids 711-1034

   Promoter

   CMV

   Available Since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_EGFR_p.G719D

   Plasmid

   #81708

   Purpose

   Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   EGFR (EGFR Human)
   

   Use

   Gateway entry vector

   Mutation

   G719D

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. RKS-Fzd7/8 subtype NGS Wnt

   Plasmid

   #159628

   Purpose

   Express Fzd7/8 subtype NGS Wnt in mammalian cells

   Depositor

   Chris Garcia

   Article

   Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

   Insert

   Fzd7/8 subtype NGS Wnt
   

   Use

   Lentiviral

   Tags

   His tag

   Expression

   Mammalian

   Available Since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.3_CoV2_501V2

   Plasmid

   #170449

   Purpose

   Express SARS-CoV-2 501V2 (South Africa strain / beta strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 501V2 pseudotyped virus production.

   Depositor

   David Nemazee

   Article

   Yuan et al Science. 2021 Aug 13;373(6556):818-823.

   Insert

   SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, 501V2 (B.1.351))
   

   Tags

   NA

   Expression

   Mammalian

   Mutation

   L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D6…

   Promoter

   CMV

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGBacmam-NGS Wnt-Fc

   Plasmid

   #159629

   Purpose

   Express Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid research

   Depositor

   Chris Garcia

   Article

   Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

   Insert

   Fzd7/8 subtype NGS Wnt Fc
   

   Tags

   Fc tag and His tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_DNMT3A_WT

   Plasmid

   #81765

   Purpose

   Gateway Donor vector containing DNMT3A , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   DNMT3A (DNMT3A Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX458-3xHA-en31FnCas9

   Plasmid

   #201949

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en31FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   G1243T, E1369R, E1603H on FnCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_SETDB1_WT

   Plasmid

   #82291

   Purpose

   Gateway Donor vector containing SETDB1, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SETDB1 (SETDB1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.3_CoV2_B.1.1.7

   Plasmid

   #170451

   Purpose

   Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.

   Depositor

   David Nemazee

   Article

   Yuan et al Science. 2021 Aug 13;373(6556):818-823.

   Insert

   SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))
   

   Tags

   NA

   Expression

   Mammalian

   Mutation

   HY69-70del, Y144del, N501Y, A570D, D614G, P681H, …

   Promoter

   CMV

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-AAV-syn-jGCaMP7s-WPRE

    Plasmid

    #104487

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP7s
    

    Use

    AAV

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_PER1_WT

    Plasmid

    #82152

    Purpose

    Gateway Donor vector containing PER1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PER1 (PER1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX458-3xHA-SpCas9-HF1

    Plasmid

    #201952

    Purpose

    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pInducer-myc-ERK1-R84S/S170D

    Plasmid

    #213593

    Purpose

    Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D cDNA in mammilian cells

    Depositor

    Channing Der

    Article

    Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

    Insert

    MAPK3 (MAPK3 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    R84S, S170D

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. HDM-SARS2-Spike-delta21

    Plasmid

    #155130

    Purpose

    Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping

    Depositor

    Jesse Bloom

    Article

    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

    Insert

    SARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Codon optimized to H. sapiens using IDT codon opt…

    Promoter

    CMV

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_ATG16L1_WT

    Plasmid

    #82255

    Purpose

    Gateway Donor vector containing ATG16L1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    ATG16L1 (ATG16L1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-N-Flag-NLRP3 91-710

    Plasmid

    #75140

    Purpose

    Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 91-710

    Promoter

    CMV

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.3_CoV2_P1

    Plasmid

    #170450

    Purpose

    Express SARS-CoV-2 P.1 (Brazil / gamma) strain spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 P.1 pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Yuan et al Science. 2021 Aug 13;373(6556):818-823.

    Insert

    SARS-CoV-2 P.1 spike D18 (S SARS-CoV-2, P.1 strain)
    

    Tags

    NA

    Expression

    Mammalian

    Mutation

    L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…

    Promoter

    CMV

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_XPO1_WT

    Plasmid

    #81812

    Purpose

    Gateway Donor vector containing XPO1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    XPO1 (XPO1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5_mEGFP-CRTC1-MAML2

    Plasmid

    #237638

    Purpose

    For overexpression of mEGFP-CRTC1-MAML2

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    CRTC1-MAML2 (CRTC1, MAML2 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHEE2E-TRI

    Plasmid

    #71288

    Purpose

    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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