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pAc5.1B-V5-SBP-FLuc_I

Plasmid

#146550

Purpose

Insect Expression of Fluc

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

Fluc

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsUPF3biso2_I

Plasmid

#146568

Purpose

Mammalian Expression of HsUPF3Biso2

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsUPF3Biso2 (UPF3B Human)

Expression

Mammalian

Mutation

one silent mutation T510C compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-HseIF4E_24-217_AF

Plasmid

#148668

Purpose

Bacterial Expression of HseIF4E_24-217

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4E_24-217 (EIF4E Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEPSW0CM0020

Plasmid

#196163

Purpose

Phytobricks Level 0 part: AtNLP6 promoter 1kb upstream

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtNLP6 promoter 1kb upstream

Use

Synthetic Biology

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0022

Plasmid

#196147

Purpose

Gateway entry vector with Arabidopsis ARF18 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtARF18_HiBit

Use

Gateway Cloning

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0012

Plasmid

#196146

Purpose

Gateway entry vector with Arabidopsis ANAC032 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtNAC032_HiBit

Use

Gateway Cloning

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0011

Plasmid

#196145

Purpose

Gateway entry vector with Arabidopsis DREB26 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtDREB26_HiBit

Use

Gateway Cloning

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0002

Plasmid

#196142

Purpose

Gateway entry vector with Arabidopsis TGA2 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtTGA2_HiBit

Use

Gateway Cloning

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0009

Plasmid

#196143

Purpose

Gateway entry vector with Arabidopsis NLP6 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtNLP6_HiBit

Use

Gateway Cloning

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYCeGM0010

Plasmid

#196144

Purpose

Gateway entry vector with Arabidopsis NLP7 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtNLP7_HiBit

Use

Gateway Cloning

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPSW0CM0014

Plasmid

#196162

Purpose

Phytobricks Level 0 part: ANAC032 promoter 1kb upstream

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

ANAC032 promoter 1kb upstream

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPSW0CM0018

Plasmid

#196164

Purpose

Phytobricks Level 0 part: AtARF18 promoter 1kb upstream

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtARF18 promoter 1kb upstream

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPSW1KN0018

Plasmid

#196176

Purpose

MoClo Level 1 luciferase reporter: proARF18::LucN::flag::notT

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

proARF18::LucN::flag::notT

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPSW1KN0020

Plasmid

#196175

Purpose

MoClo Level 1 luciferase reporter: proNLP6::LucN::flag::notT

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

proNLP6::LucN::flag::notT

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPSW1KN0014

Plasmid

#196174

Purpose

MoClo Level 1 luciferase reporter: proANAC032::LucF::flag::notT

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

proANAC032::LucF::flag::notT

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His K403A

Plasmid

#195612

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K403A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1998 Nov 13;273(46):30524-9

Insert

PI-SceI

Expression

Bacterial

Mutation

A1463G, A1464C

Promoter

T7

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pB7_9-ND51_v8_X1

Plasmid

#191511

Purpose

Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

v8 ZF-DdCBE 9-ND51 X1

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pPEPT1

Plasmid

#195001

Purpose

Production of peptides in E. coli as GB1 fusion

Depositor

Marko Hyvönen

Article

Pantelejevs et al Chem Sci. 2023 Nov 21;14(47):13915-13923

Type

Empty backbone

Tags

His8-tag and StrepTagII-GB1

Expression

Bacterial

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-ZNF354C_mutant_D17A-V18A

Plasmid

#194184

Purpose

Expresses ZNF354C with mutated KRAB domain (D17A, V18A mutation)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Oo et al Sci Rep. 2020 Nov 5;10(1):19079. doi: 10

Insert

ZNF354C (ZNF354C Human)

Tags

Myc-DDK

Expression

Mammalian

Mutation

KRAB domain mutant D17A/V18A

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-ZNF354C_mutant_E25A-W26A

Plasmid

#194186

Purpose

Expresses ZNF354C with mutated KRAB domain (E25A, W26A mutation)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Oo et al Sci Rep. 2020 Nov 5;10(1):19079. doi: 10

Insert

ZNF354C (ZNF354C Human)

Tags

Myc-DDK

Expression

Mammalian

Mutation

KRAB domain mutant E25A/W26A

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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